Stem cell-derived neurons have become important tools in neuroscience research and a number of hiPSC- derived neuronal types are currently available on the market. Culture conditions are critical for maturation of hiPSC-derived neurons and it was discovered early on that commonly used culture media, e.g. DMEM-basal, Neurobasal or serum, while promoting neuronal survival, could actually impair neurophysiological functions. In order to promote maturation of neurons, they can be co-cultured with astrocytes. In collaboration with the Fraunhofer Institute for Biomedical Engineering (IBMT) we explored co-culture of hiPSC-derived neurons (EBiSC-NEUR1) and astrocytes (ScienCell, #1800) by comparing the electrophysiological properties of the neurons using the SyncroPatch 384.