Lysosomes are essential for cellular homeostasis and their dysfunction is linked to a wide range of diseases. Given the immediate medical relevance, there is a current need for high throughput electrophysiology on lysosomes. Oria Bioscience have pioneered a novel approach for lysosomal isolation resulting in large batches of ready-to-use “LYSO-Preps” of high purity. We have successfully characterized “LYSO-Preps” overexpressing TRPML1 channels through pharmacology of activators, inhibitors and intraluminal pH sensitivity using the SyncroPatch 384, the SURFE2R N1 and the SURFE2R 96SE.