Here we present data collected on the Patchlinere showing activation of nAChRα1β1γδ expressed in human TE671 cells with rapid application of ligand. We found that ACh activates nAChRα1β1γδ receptors with an EC50 value similar to those reported in the literature. Data was acquired using the PatchlinerControl software developed for the Patchlinere and analyzed using DataControl PL, the analysis software for the Patchlinere. This software enables concentration response curves to be constructed at the click of a button and data to be pooled and averaged from multiple NPC-16 chips.