Characterization of ioSensory Neurons on the Patchliner

Human induced pluripotent stem cells (hiPSCs) are increasingly used in biomedical research for disease modeling and drug discovery. The use of stem cells for research reduces the need for animals, as well as having the added advantages of being human in origin and available in relatively high abundance. One of the challenges in the use of hiPSCs is the requirement to culture the neurons for long time periods to achieve maturity. This can pose a potential challenge when combining hiPSC-derived neurons with techniques such as automated patch clamp (APC) which require cells in suspension for the experiments. When cells are cultured for longer (4 weeks or more) this can result in lower success rates for cell capture, and therefore, overall success rate for completed experiments. bit.bio uses opti-ox (optimised inducible overexpression) technology for cell programming. With this technology, genetic programs in human stem cells can be faithfully executed, leading to precise and consistent programming of the population into a chosen cell identity at a commercial scale.