{"id":6870,"date":"2023-02-17T07:18:39","date_gmt":"2023-02-17T07:18:39","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/nav1-5-and-herg-there-is-no-f-in-apc-reliable-fluoride-free-recordings-on-the-syncropatch-384\/"},"modified":"2023-02-17T07:18:39","modified_gmt":"2023-02-17T07:18:39","slug":"nav1-5-and-herg-there-is-no-f-in-apc-reliable-fluoride-free-recordings-on-the-syncropatch-384","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/nav1-5-and-herg-there-is-no-f-in-apc-reliable-fluoride-free-recordings-on-the-syncropatch-384\/","title":{"rendered":"NaV1.5 and hERG &#8211; &#8220;There is no F in APC: Reliable fluoride-free recordings on the SyncroPatch 384&#8221;"},"content":{"rendered":"<p>Automated patch clamp (APC) instruments are used\u00a0for a wide variety of applications ranging from basic\u00a0research into channelopathies and biophysical\u00a0characteristics of ion channels, through to routine\u00a0cardiac safety testing. Their use in cardiac safety\u00a0screening has increased over the years and APC is\u00a0now an established and accepted technique in most,\u00a0if not all, safety testing laboratories. It is well known that\u00a0fluoride is often used in the internal solution in APC\u00a0experiments to improve the seal resistance. The presence\u00a0of external calcium (or other divalent cation) further\u00a0improves the seal by a mechanism thought to be due to\u00a0the formation of CaF2 crystals at the interface between\u00a0the pipette or micro-pore and the cell as described in a\u00a0recent patent application.Even in manual patch clamp\u00a0experiments, fluoride has been used to record voltage gated\u00a0Na<sup>+<\/sup> channels for over 20 years, despite known\u00a0effects on voltage dependence of conductance, and\u00a0steady-state fast inactivation and its inhibition of protein\u00a0phosphatase. Fluoride is used because it improves the seal\u00a0and allows stable measurements to be performed over\u00a0long periods of time. However, because there are some\u00a0experiments where it is advantageous to use\u00a0physiological, fluoride-free internal solutions and\u00a0external solution that does not use divalent \u2018seal\u00a0enhancer\u2019 solutions, we have developed a method that\u00a0allows fluoride-free, physiological solutions to be used\u00a0with good success rates. We demonstrate this using the\u00a0cardiac ion channels hERG expressed in HEK293 cells\u00a0(SB Drug Discovery) and NaV1.5 expressed in CHO cells\u00a0(Charles River).<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[42],"resource_type":[90],"application_areas":[50,53],"therapeutic_areas":[],"target_categories":[61],"target_families":[332,333],"targets":[92,93],"resource_keyword":[],"class_list":["post-6870","resource_library","type-resource_library","status-publish","hentry","products_tags-syncropatch-384","resource_type-application-note","application_areas-ion-channels","application_areas-assay-development-and-validation","target_categories-voltage-gated-ion-channels","target_families-nav-channels","target_families-kv-channels","targets-kv11-1-herg","targets-nav1-5"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6870","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6870"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6870"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6870"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6870"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6870"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6870"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6870"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6870"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6870"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}