{"id":6820,"date":"2023-02-17T07:07:42","date_gmt":"2023-02-17T07:07:42","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/ompf-lipid-bilayer-recordings-of-ompf-reconstituted-in-proteoliposomes\/"},"modified":"2023-02-17T07:07:42","modified_gmt":"2023-02-17T07:07:42","slug":"ompf-lipid-bilayer-recordings-of-ompf-reconstituted-in-proteoliposomes","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/ompf-lipid-bilayer-recordings-of-ompf-reconstituted-in-proteoliposomes\/","title":{"rendered":"OmpF &#8211; &#8220;Lipid Bilayer recordings of OmpF reconstituted in Proteoliposomes &#8220;"},"content":{"rendered":"<p>Solvent-free planar lipid bilayers were formed in an\u00a0automatic manner by bursting of giant unilamellar\u00a0vesicles (GUVs) positioned by suction on the apertures\u00a0of our patch clamp chips made from borosilicate\u00a0glass substrate. Incubation of GUVs with purified ion\u00a0channel protein of interest yielded proteoliposomes.\u00a0These proteoliposomes allow for immediate recording\u00a0of channel activity after GUV sealing. This approach\u00a0reduces the time consuming, laborious and\u00a0sometimes difficult protein reconstitution processes\u00a0normally performed after bilayer formation.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45,129],"resource_type":[90],"application_areas":[99],"therapeutic_areas":[],"target_categories":[341],"target_families":[349],"targets":[131],"resource_keyword":[],"class_list":["post-6820","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","products_tags-vesicle-prep-pro","resource_type-application-note","application_areas-pores-and-toxins","target_categories-pores-and-toxins","target_families-prokaryotic-pores","targets-ompf-ompc"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6820","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6820"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6820"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6820"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6820"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6820"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6820"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6820"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6820"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6820"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}