{"id":6816,"date":"2023-02-17T07:07:37","date_gmt":"2023-02-17T07:07:37","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/kcsa-lipid-bilayer-recordings-of-kcsa-channels-reconstituted-in-proteoliposomes\/"},"modified":"2023-02-17T07:07:37","modified_gmt":"2023-02-17T07:07:37","slug":"kcsa-lipid-bilayer-recordings-of-kcsa-channels-reconstituted-in-proteoliposomes","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/kcsa-lipid-bilayer-recordings-of-kcsa-channels-reconstituted-in-proteoliposomes\/","title":{"rendered":"KcsA &#8211; &#8220;Lipid bilayer recordings of KcsA channels reconstituted in proteoliposomes&#8221;"},"content":{"rendered":"<p>Solvent-free planar lipid bilayers were formed in an\u00a0automated manner using suction to attract a giant\u00a0unilamellar vesicle (GUV) to the patch clamp chip which\u00a0subsequently bursts across the aperture. Incubation of GUV&#8217;s with purified KcsA channel protein yielded\u00a0proteoliposomes. These proteoliposomes allow for\u00a0immediate recording of channel activity after GUV\u00a0sealing. The rapid formation of protein-containing planar lipid bilayers is of potential use for the efficient electrophysiological characterization of KcsA as shown\u00a0here and also other ion channel proteins of interest.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45,129],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[],"target_categories":[61,338],"target_families":[350],"targets":[130],"resource_keyword":[],"class_list":["post-6816","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","products_tags-vesicle-prep-pro","resource_type-application-note","application_areas-ion-channels","target_categories-voltage-gated-ion-channels","target_categories-other-ion-channels","target_families-prokaryotic-tetrameric-cation-channels","targets-ph-gated-potassium-channel-kcsa"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6816","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6816"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6816"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6816"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6816"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6816"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6816"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6816"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6816"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6816"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}