{"id":6802,"date":"2023-02-17T07:05:11","date_gmt":"2023-02-17T07:05:11","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/nav1-7-patch-clamp-recordings-of-hnav1-7-on-nanions-port-a-patch\/"},"modified":"2023-02-17T07:05:13","modified_gmt":"2023-02-17T07:05:13","slug":"nav1-7-patch-clamp-recordings-of-hnav1-7-on-nanions-port-a-patch","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/nav1-7-patch-clamp-recordings-of-hnav1-7-on-nanions-port-a-patch\/","title":{"rendered":"NaV1.7 &#8211; &#8220;Patch clamp recordings of hNaV1.7 on Nanion\u2019s Port-a-Patch&#8221;"},"content":{"rendered":"<p>The NaV1.7 gene (SCN9A) encodes a voltage-gated sodium (NaV) channel, primarily expressed in the peripheral nervous system and has been isolated from rat dorsal root ganglion (DRG) neurons, human medullary thyroid cancer cells (hNE-Na) \u00a0and PC12 cells.\u00a0Different NaV\u00a0channels play a key role in modulation of action potentials in the central and peripheral nervous systems. In particular, the fast upstroke of the action potential is mediated by NaV\u00a0channels. NaV\u00a0channels are in part characterized by their TTX-sensitivity (TTX-resistant [TTXr], TTX-sensitive [TTXs]). NaV1.7 is a TTXs channel and is sensitive to TTX in the \u00a0nanomolar range. The role of hNaV1.7 has yet to be fully elucidated but is proposed to play an important role in nociception and pain sensing. NaV1.7 has been\u00a0implicated to play a role in disease pain states, in particular inflammatory pain and hypersensitivity to heat following burn injury. Common to many of the voltagegated ion channels, a number of compounds display a \u00a0higher affinity for the inactivated state of the channel. For this reason, it is important to be able to reliably record both activation and inactivation kinetics of the channel.\u00a0In this Application Note we present data using the Port-a-Patch characterizing CHO cells stably expressing hNaV1.7. The hNaV1.7 activation and\u00a0inactivation properties and TTX sensitivity are consistent with those reported in the literature.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[55,56],"target_categories":[61],"target_families":[332],"targets":[116],"resource_keyword":[],"class_list":["post-6802","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","resource_type-application-note","application_areas-ion-channels","therapeutic_areas-cns","therapeutic_areas-pain","target_categories-voltage-gated-ion-channels","target_families-nav-channels","targets-nav1-7"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6802","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6802"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6802"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6802"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6802"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6802"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6802"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6802"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6802"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6802"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}