{"id":6794,"date":"2023-02-17T07:04:57","date_gmt":"2023-02-17T07:04:57","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/herg-temperature-controlled-herg-recordings-on-the-port-a-patch\/"},"modified":"2023-02-17T07:04:59","modified_gmt":"2023-02-17T07:04:59","slug":"herg-temperature-controlled-herg-recordings-on-the-port-a-patch","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/herg-temperature-controlled-herg-recordings-on-the-port-a-patch\/","title":{"rendered":"hERG &#8211; &#8220;Temperature controlled hERG recordings on the Port-a-Patch&#8221;"},"content":{"rendered":"<p>The hERG gene encodes a potassium channel\u00a0responsible for the repolarization of the I<sup>Kr<\/sup> current in\u00a0cardiac cells. This channel is important in the repolarization\u00a0of the cardiac action potential. Abnormalities\u00a0in this channel can cause long or\u00a0short QT syndrome, leading to potentially fatal\u00a0cardiac arrhythmia. Given the importance of this\u00a0channel in maintaining cardiac function, and\u00a0disturbances of channel activity by certain compounds\u00a0such as anti-arrhythmias and anti-psychotics, it has\u00a0become an important target in compound safety\u00a0screening. It is a desirable option to study this channel\u00a0at physiological temperature since compounds can\u00a0display different actions or potencies at physiological\u00a0temperature.\u00a0Here, we present data collected on the Port-a-Patch\u00a0using the External Perfusion System coupled with Nanion\u2019s\u00a0Temperature Control. Cells were captured and sealed\u00a0at room temperature and control recordings made before\u00a0raising the temperature to 35\u00b0C so that parameters\u00a0such as current peak amplitude could be compared at\u00a0the two different temperatures. Furthermore, the hERG\u00a0active compound, quinidine, was used at physiological\u00a0temperature and a full dose response curve was\u00a0achieved. This demonstrates the stability of the\u00a0recordings on the Port-a-Patch at this temperature. The\u00a0concentration response curve generated an IC<sub>50<\/sub>\u00a0similar\u00a0to that obtained at room temperature, and similar to that\u00a0published in the literature.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[54],"target_categories":[61],"target_families":[333],"targets":[92],"resource_keyword":[],"class_list":["post-6794","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","resource_type-application-note","application_areas-ion-channels","therapeutic_areas-cardiac-safety-cipa","target_categories-voltage-gated-ion-channels","target_families-kv-channels","targets-kv11-1-herg"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6794","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6794"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6794"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6794"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6794"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6794"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6794"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6794"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6794"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6794"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}