{"id":6790,"date":"2023-02-17T07:04:52","date_gmt":"2023-02-17T07:04:52","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/herg-characterization-of-herg-hek293-on-nanions-port-a-patch\/"},"modified":"2023-02-17T07:04:54","modified_gmt":"2023-02-17T07:04:54","slug":"herg-characterization-of-herg-hek293-on-nanions-port-a-patch","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/herg-characterization-of-herg-hek293-on-nanions-port-a-patch\/","title":{"rendered":"hERG &#8211; &#8220;Characterization of hERG (HEK293) on Nanion&#8217;s Port-a-Patch&#8221;"},"content":{"rendered":"<p>The hERG gene (KCNH2) encodes a potassium ion channel\u00a0responsible for the repolarizing I<sup>Kr<\/sup>\u00a0current in the cardiac\u00a0action potential (Sanguinetti et al., 1995).\u00a0Abnormalities in this channel may lead to either Long QT\u00a0Syndrome (LQT2) (with loss-of-function mutations) or Short\u00a0QT syndrome (with gain-of-function mutations), both potentially\u00a0fatal cardiac arrhythmia, due to repolarization\u00a0disturbances of the cardiac action potential.\u00a0Given the importance of this channel in maintaining cardiac\u00a0function, it has become an important target in compound\u00a0safety screening.\u00a0A large range of therapeutic agents with diverse chemical\u00a0structures have been reported to induce long QT syndrome.\u00a0These include antihistamines (e.g. terfenadine),\u00a0gastrointestinal prokinetic agents (e.g. cisapride) and\u00a0others.\u00a0In this report we present data that were collected on the\u00a0Port-a-Patch. Cells (HEK293 stably expressing hERG, supplied\u00a0by Millipore) were tested. Current amplitudes, IVs\u00a0and cisapride as\u00a0well as quinidine dose-response curves were analyzed.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[54],"target_categories":[61],"target_families":[333],"targets":[92],"resource_keyword":[],"class_list":["post-6790","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","resource_type-application-note","application_areas-ion-channels","therapeutic_areas-cardiac-safety-cipa","target_categories-voltage-gated-ion-channels","target_families-kv-channels","targets-kv11-1-herg"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6790","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6790"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6790"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6790"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6790"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6790"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6790"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6790"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6790"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6790"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}