{"id":6768,"date":"2023-02-17T06:59:24","date_gmt":"2023-02-17T06:59:24","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/p2x3-activation-and-inhibition-of-p2x3-channels-recorded-on-the-patchliner\/"},"modified":"2023-02-17T06:59:24","modified_gmt":"2023-02-17T06:59:24","slug":"p2x3-activation-and-inhibition-of-p2x3-channels-recorded-on-the-patchliner","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/p2x3-activation-and-inhibition-of-p2x3-channels-recorded-on-the-patchliner\/","title":{"rendered":"P2X3 &#8211; &#8220;Activation and inhibition of P2X3 channels recorded on the Patchliner&#8221;"},"content":{"rendered":"<p>P2X receptors are ligand-gated ion channels that open in response to extracellular ATP. They are permeable to small monovalent cations, some having significant divalent or anion permeability. P2X receptors are found on many cell types including smooth muscle cells, sensory neurones, epithelia, bone and leukocytes. A role for P2X receptors has been suggested in transmission of thermal stimuli, chemosensory signalling, taste and pain. To date, 7 P2X receptor genes have been cloned and studied in heterologous expression systems. Functional receptors are trimeric, which can be homomeric or heteromeric. The P2X2\u00a0and P2X3\u00a0receptors can function either as homomers or as P2X2\/3\u00a0heteromers. When expressed together, a mixture of P2X2\u00a0and P2X3\u00a0homomers as well as P2X2\/3\u00a0heteromers are likely to exist, which may be distinguished through their biophysical and pharmacological properties. Both P2X3\u00a0homomers and P2X2\/3\u00a0heteromeric receptors have been implicated in nociception and pain signalling and may be important therapeutic targets for analgesic drugs. The P2X3\u00a0and P2X2\/3\u00a0receptor antagonist MK-7264 (gefapixant), has recently progressed to Phase III trials for refractory or unexplained chronic cough.Here we present data collected on the Patchliner showing activation and inhibition of P2X3\u00a0currents expressed in CHO cells with rapid and brief application of ligand (using the stacked solution approach). \u03b1\u03b2-methylene ATP (\u03b1\u03b2-MeATP) activated P2X3 receptors in a concentrationdependent manner with an EC<sub>50<\/sub> value similar to those in the literature. P2X3\u00a0receptors could be repetitively activated by \u03b1\u03b2-MeATP and blocked by A-317491 with an IC<sub>50<\/sub>\u00a0value in good agreement with the literature.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[44],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[55,56],"target_categories":[337],"target_families":[358],"targets":[119],"resource_keyword":[],"class_list":["post-6768","resource_library","type-resource_library","status-publish","hentry","products_tags-patchliner","resource_type-application-note","application_areas-ion-channels","therapeutic_areas-cns","therapeutic_areas-pain","target_categories-ligand-gated-ion-channels","target_families-p2x-receptors","targets-p2x3"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6768","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6768"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6768"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6768"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6768"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6768"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6768"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6768"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6768"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6768"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}