{"id":6762,"date":"2023-02-17T06:59:14","date_gmt":"2023-02-17T06:59:14","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/neurons-peri-4u-and-dopa-4u-stem-cell-derived-neurons-recorded-on-nanions-patchliner\/"},"modified":"2023-02-17T06:59:16","modified_gmt":"2023-02-17T06:59:16","slug":"neurons-peri-4u-and-dopa-4u-stem-cell-derived-neurons-recorded-on-nanions-patchliner","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/neurons-peri-4u-and-dopa-4u-stem-cell-derived-neurons-recorded-on-nanions-patchliner\/","title":{"rendered":"Neurons &#8211; &#8220;Peri.4U and Dopa.4U stem cell-derived neurons recorded on Nanion\u00b4s Patchliner&#8221;"},"content":{"rendered":"<p>Human induced pluripotent stem cell-derived neurons\u00a0(hiPSC-neurons) may provide a viable cellular model\u00a0for studying the mechanisms underlying neurological\u00a0diseases and drug development. Axiogenesis provides\u00a0a number of hiPSC-neurons including dopaminergic\u00a0neurons (Dopa.4U) and peripheral neurons (Peri.4U),\u00a0amongst others. These neurons have been used on\u00a0in-vitro systems such as multielectrode arrays (MEA),\u00a0immunocytochemistry and calcium imaging. They are\u00a0an interesting model for studying neurological diseases\u00a0such as Parkinson\u2019s Disease, as well as for efficacy, drug\u00a0discovery and toxicity studies.\u00a0In this study, the Patchliner was used to record from\u00a0Dopa.4U and Peri.4U neurons in voltage and current\u00a0clamp modes. The cell harvesting procedure was\u00a0optimized (using papain) to ensure that the cells retained\u00a0proximal dendrites and initial axon segments in order to\u00a0maintain ion channel expression present in these regions.\u00a0Due to their irregular shape (presence of processes),\u00a0success rate (typically 20 &#8211; 50% for RSeal >200 M\u2126) was\u00a0lower than other cell types which have a smooth, round\u00a0shape, e.g. standard cell lines. Voltage-gated Na<sup>+<\/sup>\u00a0(NaV)\u00a0and K<sup>+<\/sup>\u00a0(KV) currents were recorded in both cell types.\u00a0Action potentials (AP) were also recorded and block of\u00a0the AP of Dopa.4U cells by lidocaine is shown.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[44],"resource_type":[90],"application_areas":[50,77],"therapeutic_areas":[55],"target_categories":[61],"target_families":[332,333],"targets":[116],"resource_keyword":[],"class_list":["post-6762","resource_library","type-resource_library","status-publish","hentry","products_tags-patchliner","resource_type-application-note","application_areas-ion-channels","application_areas-cell-ipscs-profiling","therapeutic_areas-cns","target_categories-voltage-gated-ion-channels","target_families-nav-channels","target_families-kv-channels","targets-nav1-7"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6762","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6762"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6762"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6762"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6762"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6762"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6762"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6762"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6762"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6762"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}