{"id":6693,"date":"2023-02-17T06:58:27","date_gmt":"2023-02-17T06:58:27","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/nav1-5-late-ina-late-recorded-from-cho-cells-and-hipsc-cms-on-nanions-patchliner\/"},"modified":"2023-02-17T06:58:27","modified_gmt":"2023-02-17T06:58:27","slug":"nav1-5-late-ina-late-recorded-from-cho-cells-and-hipsc-cms-on-nanions-patchliner","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/nav1-5-late-ina-late-recorded-from-cho-cells-and-hipsc-cms-on-nanions-patchliner\/","title":{"rendered":"NaV1.5-Late &#8211; &#8220;INa-Late recorded from CHO cells and hiPSC-CMs on Nanion\u00b4s Patchliner&#8221;"},"content":{"rendered":"<p>The voltage-gated Na<sup>+<\/sup>\u00a0channel 1.5 (NaV1.5) is encoded by the SCN5A gene and is responsible for the rising phase of the cardiac action potential (AP). The NaV1.5 channel is comprised of a pore-forming \u03b1 subunit and auxillary \u03b2 subunits. When the cardiac cell membrane depolarizes, NaV1.5 opens for a short time allowing an influx of Na<sup>+<\/sup>\u00a0ions resulting in the upstroke of the AP. During the AP, these channels can recover from inactivation and re-open resulting in a sustained current termed INa-Late. Although this current is substantially smaller than the peak Na<sup>+<\/sup>\u00a0current (INa-Peak), it is active during the plateau phase and therefore contributes to AP morphology. There is a growing body of evidence that increased INa-Late\u00a0can have a pathophysiological role in acquired heart diseases such as myocardial ischemia and heart failure. INa-Late\u00a0is elevated in several pathological conditions which could result in Na<sup>+<\/sup>-overload in these cells. A number of loss or gain-of-function mutations in the SCN5A gene have been identified which lead to changes in the magnitude or duration of INa-Peak\u00a0or INa-Late\u00a0resulting in fatal arrhythmias. INa-Late\u00a0is a potential drug target to treat cardiac disorders such as angina, heart failure and arrhythmia. It is also an important target in safety pharmacology as enhancement of INa-Late\u00a0is proarrhythmic.In this study the Patchliner was used to record INa-Late\u00a0from CHO cells and hiPSC-CMs. INa-Late\u00a0was recorded using the voltage protocol specified by CiPA and activated using ATX-II. INa-Late\u00a0could be recorded from CHO cells stably expressing NaV1.5 and blocked by lidocaine. INa-Late\u00a0could also be detected in iCell<sup>\u00ae<\/sup>\u00a0Cardiomyoctes2\u00a0and blocked by ranolazine.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[44],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[54],"target_categories":[61],"target_families":[332],"targets":[93],"resource_keyword":[],"class_list":["post-6693","resource_library","type-resource_library","status-publish","hentry","products_tags-patchliner","resource_type-application-note","application_areas-ion-channels","therapeutic_areas-cardiac-safety-cipa","target_categories-voltage-gated-ion-channels","target_families-nav-channels","targets-nav1-5"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6693","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6693"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6693"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6693"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6693"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6693"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6693"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6693"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6693"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6693"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}