{"id":6550,"date":"2023-02-17T07:11:30","date_gmt":"2023-02-17T07:11:30","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2021-structural-basis-for-potassium-transport-by-kdpfabc\/"},"modified":"2023-02-17T07:11:31","modified_gmt":"2023-02-17T07:11:31","slug":"2021-structural-basis-for-potassium-transport-by-kdpfabc","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2021-structural-basis-for-potassium-transport-by-kdpfabc\/","title":{"rendered":"2021 &#8211; Structural basis for Potassium transport by KdpFABC"},"content":{"rendered":"<p>KdpFABC is an oligomeric K<sup>+<\/sup> transport complex in prokaryotes that maintains ionic homeostasis\u00a0under stress conditions. The complex comprises a channel-like subunit (KdpA) from the\u00a0Superfamily of K<sup>+<\/sup> Transporters and a pump-like subunit (KdpB) from the superfamily of P-type\u00a0ATPases. Recent structural work has defined the architecture and generated contradictory\u00a0hypotheses for the transport mechanism. Here, we use substrate analogs to stabilize four key\u00a0intermediates in the reaction cycle and determine the corresponding structures by cryo-EM.\u00a0We find that KdpB undergoes conformational changes consistent with other representatives\u00a0from the P-type superfamily, whereas KdpA, KdpC and KdpF remain static. We observe a series\u00a0of spherical densities that we assign as K<sup>+<\/sup> or water and which define a pathway for K<sup>+<\/sup> transport.\u00a0This pathway runs through an intramembrane tunnel in KdpA and delivers ions to sites in the\u00a0membrane domain of KdpB. Our structures suggest a mechanism where ATP hydrolysis is\u00a0coupled to K<sup>+<\/sup> transfer between alternative sites in the membrane domain of KdpB, ultimately\u00a0reaching a low-affinity site where a water-filled pathway allows release of K<sup>+<\/sup> to the cytoplasm.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[108],"therapeutic_areas":[],"target_categories":[60],"target_families":[344],"targets":[208],"resource_keyword":[],"class_list":["post-6550","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-transporters","target_categories-transporters","target_families-p-type-atpases","targets-na-k-atpase"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6550","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6550"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6550"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6550"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6550"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6550"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6550"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6550"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6550"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6550"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}