{"id":6419,"date":"2023-02-17T07:03:54","date_gmt":"2023-02-17T07:03:54","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2020-homocysteine-induced-electrical-remodeling-via-the-mediation-of-ip-3-r1-nav1-5-signaling-pathway\/"},"modified":"2023-02-17T07:03:54","modified_gmt":"2023-02-17T07:03:54","slug":"2020-homocysteine-induced-electrical-remodeling-via-the-mediation-of-ip-3-r1-nav1-5-signaling-pathway","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2020-homocysteine-induced-electrical-remodeling-via-the-mediation-of-ip-3-r1-nav1-5-signaling-pathway\/","title":{"rendered":"2020 &#8211; Homocysteine-induced electrical remodeling via the mediation of IP 3 R1\/NaV1.5 signaling pathway"},"content":{"rendered":"<p>Inositol-1,4,5-triphosphate-receptor 1 (IP3R1), a Ca<sup>2+<\/sup> channel in the sarcoplasmic reticulum membrane, is an effective regulator of Ca<sup>2+<\/sup> release involved in the pathology of most cardiovascular diseases. Our study aim to investigate the underlying mechanism by which IP3R1 signaling mediates the process of homocysteine (Hcy)-induced Ca<sup>2+<\/sup> accumulation via interaction with sodium current (NaV1.5) in atrium. We utilized whole-cell patch-clamp analysis and flow cytometry to detect the abnormal electrical activity in mouse atrial myocytes (MACs) obtained from C57B6 mice fed with high-Hcy diet. The results represented not only an increase in protein levels of NaV1.5 and IP3R1, but also an enhanced intracellular levels of Ca<sup>2+<\/sup>, and prolonged action potential duration (APD). However, the inhibition of IP3R1 or NaV1.5 gene could both attenuate Ca<sup>2+<\/sup> accumulation in MACs triggered by Hcy, as well as abnormal electrical activity. In addition, Hcy increased the interaction between IP3R1 and NaV1.5. These data suggest that Hcy induced Ca<sup>2+<\/sup> accumulation is mediated by the IP3R1\/NaV1.5 signaling pathway, accompanied with the influx of Na<sup>+<\/sup> and Ca<sup>2+<\/sup>, which act as triggers for electrical remodeling.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45],"resource_type":[75],"application_areas":[50,108],"therapeutic_areas":[],"target_categories":[61,60],"target_families":[332,344],"targets":[93,207],"resource_keyword":[],"class_list":["post-6419","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","resource_type-publication","application_areas-ion-channels","application_areas-transporters","target_categories-voltage-gated-ion-channels","target_categories-transporters","target_families-nav-channels","target_families-p-type-atpases","targets-nav1-5","targets-serca"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6419","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6419"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6419"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6419"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6419"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6419"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6419"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6419"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6419"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6419"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}