{"id":6212,"date":"2023-02-17T07:06:52","date_gmt":"2023-02-17T07:06:52","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2017-the-rotavirus-nsp4-viroporin-domain-is-a-calcium-conducting-ion-channel\/"},"modified":"2023-02-17T07:06:53","modified_gmt":"2023-02-17T07:06:53","slug":"2017-the-rotavirus-nsp4-viroporin-domain-is-a-calcium-conducting-ion-channel","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2017-the-rotavirus-nsp4-viroporin-domain-is-a-calcium-conducting-ion-channel\/","title":{"rendered":"2017 &#8211; The Rotavirus NSP4 Viroporin Domain is a Calcium-conducting Ion Channel"},"content":{"rendered":"<p>Viroporins are small virus-encoded ion channel proteins. Most viroporins are monovalent selective cation channels, with few showing the ability to conduct divalent cations, like calcium (Ca<sup>2+<\/sup>). Nevertheless, some viroporins are known to disrupt host cell Ca<sup>2+<\/sup>\u00a0homeostasis, which is critical for virus replication and pathogenesis. Rotavirus nonstructural protein 4 (NSP4) is an endoplasmic reticulum transmembrane glycoprotein that has a viroporin domain (VPD), and NSP4 viroporin activity elevates cytosolic Ca<sup>2+<\/sup>\u00a0in mammalian cells. The goal of this study was to demonstrate that the NSP4 VPD forms an ion channel and determine whether the channel can conduct Ca<sup>2+<\/sup>. Using planar lipid bilayer and liposome patch clamp electrophysiology, we show that a synthetic peptide of the NSP4 VPD has ion channel activity. The NSP4 VPD was selective for cations over anions and channel activity was observed to have both well-defined \u201csquare top\u201d openings as well as fast current fluctuations, similar to other viroporins. Importantly, the NSP4 VPD showed similar conductance of divalent cations (Ca<sup>2+<\/sup>\u00a0and B<sup>a2+<\/sup>) as monovalent cations (K<sup>+<\/sup>), but a viroporin defective mutant lacked Ca<sup>2+<\/sup>\u00a0conductivity. These data demonstrate that the NSP4 VPD is a Ca<sup>2+<\/sup>-conducting viroporin and establish the mechanism by which NSP4 disturbs host cell Ca<sup>2+<\/sup>\u00a0homeostasis.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45,129],"resource_type":[75],"application_areas":[99],"therapeutic_areas":[],"target_categories":[341],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-6212","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","products_tags-vesicle-prep-pro","resource_type-publication","application_areas-pores-and-toxins","target_categories-pores-and-toxins"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6212","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6212"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6212"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6212"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6212"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6212"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6212"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6212"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6212"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6212"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}