{"id":6188,"date":"2023-02-17T07:06:48","date_gmt":"2023-02-17T07:06:48","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2017-evaluation-of-the-antimicrobial-activity-of-the-mastoparan-polybia-mpii-isolated-from-venom-of-the-social-wasp-pseudopolybia-vespiceps-testacea-vespidae-hymenoptera\/"},"modified":"2023-02-17T07:06:48","modified_gmt":"2023-02-17T07:06:48","slug":"2017-evaluation-of-the-antimicrobial-activity-of-the-mastoparan-polybia-mpii-isolated-from-venom-of-the-social-wasp-pseudopolybia-vespiceps-testacea-vespidae-hymenoptera","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2017-evaluation-of-the-antimicrobial-activity-of-the-mastoparan-polybia-mpii-isolated-from-venom-of-the-social-wasp-pseudopolybia-vespiceps-testacea-vespidae-hymenoptera\/","title":{"rendered":"2017 &#8211; Evaluation of the antimicrobial activity of the mastoparan Polybia-MPII isolated from venom of the social wasp Pseudopolybia vespiceps testacea (Vespidae, Hymenoptera)"},"content":{"rendered":"<p>Mastoparans, a class of peptides found in wasp venom, have significant effects following a sting as well as useful applications in clinical practice. Among these is their potential use in the control of micro-organisms that cause infectious diseases with a significant impact on society. Thus, the present study describes the isolation and identification of a mastoparan peptide from the venom of the social wasp\u00a0Pseudopolybia vespiceps\u00a0and evaluated its antimicrobial profile against bacteria (Staphylococcus aureus\u00a0and\u00a0Mycobacterium abscessus\u00a0subsp.\u00a0massiliense), fungi (Candida albicans\u00a0and\u00a0Cryptococcus neoformans) and in vivo\u00a0S. aureus\u00a0infection. The membrane pore-forming ability was also assessed. The mastoparan reduced in vitro and ex vivo mycobacterial growth by 80% at 12.5\u2009\u00b5M in infected peritoneal macrophages but did not affect the shape of bacterial cells at the dose tested (6.25\u2009\u00b5M). The peptide also showed potent action against\u00a0S. aureus\u00a0in vitro (EC<sub>50<\/sub>\u00a0and EC90\u00a0values of 1.83\u2009\u00b5M and 2.90\u2009\u00b5M, respectively) and reduced the in vivo bacterial load after 6 days of topical treatment (5\u2009mg\/kg). Antifungal activity was significant, with EC<sub>50<\/sub>\u00a0and EC90\u00a0values of 12.9\u2009\u00b5M and 15.3\u2009\u00b5M, respectively, for\u00a0C. albicans, and 11\u2009\u00b5M and 22.70\u2009\u00b5M, respectively, for\u00a0C. neoformans. Peptides are currently attracting interest for their potential in the design of antimicrobial drugs, particularly due to the difficulty of micro-organisms in developing resistance to them. In this respect, Polybia-MPII proved to be highly effective, with a lower haemolysis rate compared with peptides of the same family.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45,129],"resource_type":[75],"application_areas":[99],"therapeutic_areas":[],"target_categories":[341],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-6188","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","products_tags-vesicle-prep-pro","resource_type-publication","application_areas-pores-and-toxins","target_categories-pores-and-toxins"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6188","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6188"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6188"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6188"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6188"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6188"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6188"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6188"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6188"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6188"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}