{"id":6112,"date":"2023-02-17T07:06:27","date_gmt":"2023-02-17T07:06:27","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2014-temperature-sensitive-gating-of-hcx26-high-resolution-raman-spectroscopy-sheds-light-on-conformational-changes\/"},"modified":"2023-02-17T07:06:27","modified_gmt":"2023-02-17T07:06:27","slug":"2014-temperature-sensitive-gating-of-hcx26-high-resolution-raman-spectroscopy-sheds-light-on-conformational-changes","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2014-temperature-sensitive-gating-of-hcx26-high-resolution-raman-spectroscopy-sheds-light-on-conformational-changes\/","title":{"rendered":"2014 &#8211; Temperature-sensitive gating of hCx26: high-resolution Raman spectroscopy sheds light on conformational changes"},"content":{"rendered":"<p>The temperature-sensitive gating of human Connexin 26 (hCx26) was analyzed with confocal Raman microscopy. High-resolution Raman spectra covering the spectral range between 400 and 1500 rel. cm\u22121\u00a0with a spectral resolution of 1 cm\u22121\u00a0were fully annotated, revealing notable differences between the spectrum recorded from solubilized hCx26 in Ca<sup>2+<\/sup>-buffered POPC at 10\u00b0C and any other set of protein conditions (temperature, Ca<sup>2+<\/sup>\u00a0presence, POPC presence). Spectral components originating from specific amino acids show that the TM1\/EL1 parahelix and probably the TM4 trans-membrane helix and the plug domain are involved in the gating process responsible for fully closing the hemichannel.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45,129],"resource_type":[75],"application_areas":[99],"therapeutic_areas":[],"target_categories":[341],"target_families":[342],"targets":[227],"resource_keyword":[],"class_list":["post-6112","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","products_tags-vesicle-prep-pro","resource_type-publication","application_areas-pores-and-toxins","target_categories-pores-and-toxins","target_families-connexins","targets-cx26"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6112","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6112"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6112"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6112"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6112"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6112"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6112"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6112"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6112"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6112"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}