{"id":6098,"date":"2023-02-17T07:10:39","date_gmt":"2023-02-17T07:10:39","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2014-keeping-it-simple-transport-mechanism-and-ph-regulation-in-na-h-exchangers\/"},"modified":"2023-02-17T07:10:39","modified_gmt":"2023-02-17T07:10:39","slug":"2014-keeping-it-simple-transport-mechanism-and-ph-regulation-in-na-h-exchangers","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2014-keeping-it-simple-transport-mechanism-and-ph-regulation-in-na-h-exchangers\/","title":{"rendered":"2014 &#8211; Keeping it simple, transport mechanism and pH regulation in Na<sup>+<\/sup>\/H+ exchangers"},"content":{"rendered":"<p>Na<sup>+<\/sup>\/H+\u00a0exchangers are essential for regulation of intracellular proton and sodium concentrations in all living organisms. We examined and experimentally verified a kinetic model for Na<sup>+<\/sup>\/H+\u00a0exchangers, where a single binding site is alternatively occupied by Na<sup>+<\/sup>\u00a0or one or two H+\u00a0ions. The proposed transport mechanism inherently down-regulates Na<sup>+<\/sup>\/H+\u00a0exchangers at extreme pH, preventing excessive cytoplasmic acidification or alkalinization. As an experimental test system we present the first electrophysiological investigation of an electroneutral Na<sup>+<\/sup>\/H+\u00a0exchanger, NhaP1 from Methanocaldococcus jannaschii (MjNhaP1), a close homologue of the medically important eukaryotic NHE Na<sup>+<\/sup>\/H+\u00a0exchangers. The kinetic model describes the experimentally observed substrate dependences of MjNhaP1, and the transport mechanism explains alkaline down-regulation of MjNhaP1. Because this model also accounts for acidic down-regulation of the electrogenic NhaA Na<sup>+<\/sup>\/H+\u00a0exchanger from Escherichia coli (EcNhaA, shown in a previous publication) we conclude that it applies generally to all Na<sup>+<\/sup>\/H+\u00a0exchangers, electrogenic as well as electroneutral, and elegantly explains their pH regulation. Furthermore, the electrophysiological analysis allows insight into the electrostatic structure of the translocation complex in electroneutral and electrogenic Na<sup>+<\/sup>\/H+\u00a0exchangers.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[108],"therapeutic_areas":[],"target_categories":[60],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-6098","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-transporters","target_categories-transporters"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/6098","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=6098"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=6098"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=6098"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=6098"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=6098"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=6098"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=6098"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=6098"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=6098"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}