{"id":4868,"date":"2023-02-17T06:56:00","date_gmt":"2023-02-17T06:56:00","guid":{"rendered":"http:\/\/ionchanneltargets.com\/resource_library\/nachra4s2-pharmacology-of-human-a4s2-nachr-recorded-on-the-syncropatch-384pe\/"},"modified":"2023-02-17T06:56:00","modified_gmt":"2023-02-17T06:56:00","slug":"2012-characterizing-human-ion-channels-in-induced-pluripotent-stem-cell-derived-neurons","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2012-characterizing-human-ion-channels-in-induced-pluripotent-stem-cell-derived-neurons\/","title":{"rendered":"2012 &#8211; Characterizing Human Ion Channels in Induced Pluripotent Stem Cell-Derived Neurons"},"content":{"rendered":"<p>Neurons derived from human-induced pluripotent stem cells were characterized using manual and automated patch-clamp recordings. These cells expressed voltage-gated Na<sup>+<\/sup>\u00a0(NaV), Ca<sup>2+<\/sup>\u00a0(CaV), and K<sup>+<\/sup>\u00a0(KV) channels as expected from excitable cells. The NaV\u00a0current was TTX sensitive, IC<sub>50<\/sub>\u00a0= 12 \u00b1 6 nM (n\u00a0= 5). About 50% of the CaV\u00a0current was blocked by 10 \u00b5M of the L-type channel blocker nifedipine. Two populations of the KV\u00a0channel were present in different proportions: an inactivating (A-type) and a noninactivating type. The A-type current was sensitive to 4-AP and TEA (IC<sub>50<\/sub>\u00a0= 163 \u00b1 93 \u00b5M;\u00a0n\u00a0= 3). Application of \u03b3-aminobutyric acid (GABA) activated a current sensitive to the GABAA\u00a0receptor antagonist bicuculline, IC<sub>50<\/sub>\u00a0= 632 \u00b1 149 nM (n\u00a0= 5). In both devices, comparable action potentials were generated in the current clamp. With unbiased, automated patch clamp, about 40% of the cells expressed NaV\u00a0currents, whereas visual guidance in manual patch clamp provided almost a 100% success rate of patching \u201cexcitable cells.\u201d These results show high potential for pluripotent stem cell\u2013derived neurons as a useful model for drug discovery, in combination with automated patch-clamp recordings for high-throughput and high-quality drug assessments at human neuronal ion channels in their correct cellular background.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[44],"resource_type":[75],"application_areas":[50,77],"therapeutic_areas":[],"target_categories":[61,337],"target_families":[332,333,359],"targets":[190,192,215],"resource_keyword":[],"class_list":["post-4868","resource_library","type-resource_library","status-publish","hentry","products_tags-patchliner","resource_type-publication","application_areas-ion-channels","application_areas-cell-ipscs-profiling","target_categories-voltage-gated-ion-channels","target_categories-ligand-gated-ion-channels","target_families-nav-channels","target_families-kv-channels","target_families-gabaa-receptors","targets-gabaa-1-2-2","targets-gabaa-5-3-2","targets-gabaa-1-3-2"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/4868","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=4868"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=4868"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=4868"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=4868"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=4868"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=4868"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=4868"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=4868"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=4868"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}