{"id":4817,"date":"2023-02-17T07:01:45","date_gmt":"2023-02-17T07:01:45","guid":{"rendered":"http:\/\/ionchanneltargets.com\/resource_library\/mscl-lipid-bilayer-recordings-of-a-mechanosensitive-channel-mscl-using-nanion%c2%92s-pressure-clampchannel-mscl-using-nanion%c2%92s-pressure-clamp\/"},"modified":"2023-02-17T07:01:45","modified_gmt":"2023-02-17T07:01:45","slug":"2010-trpv-channels-mediate-temperature-sensing-in-human-corneal-endothelial-cells","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2010-trpv-channels-mediate-temperature-sensing-in-human-corneal-endothelial-cells\/","title":{"rendered":"2010 &#8211; TRPV channels mediate temperature-sensing in human corneal endothelial cells"},"content":{"rendered":"<p>The physiology and transparency of the cornea are dependent on corneal endothelial function. The role of temperature sensitive ion channels in maintaining such activity is unknown. This study was undertaken to probe for the functional expression of such pathways in human corneal endothelial cells (HCEC). We used HCEC-12, an immortalized population derived from whole corneal endothelium, and two morphologically distinct clonal cell lines derived from HCEC-12 (HCEC-H9C1, HCEC-B4G12) to probe for gene expression and function of transient receptor potential (TRP) channels of the vanilloid (V) isoform subfamily (i.e. TRPV1\u20133) in these cell types. Expression of TRPV isotypes 1, 2 and 3 were detected by RT-PCR. Protein expression of TRPV1 in situ was confirmed by immunostaining of corneoscleral remnants after keratoplasty. TRPV1\u20133 functional activity was evident based on capsaicin-induced Ca<sup>2+<\/sup>\u00a0transients and induction of these responses through rises in ambient temperature from 25\u00a0\u00b0C to over 40\u00a0\u00b0C. The currents underlying Ca<sup>2+<\/sup>\u00a0transients were characterized with a novel high throughput patch-clamp system. The TRPV1 selective agonist, capsaicin (CAP) (10\u201320\u00a0\u03bcM) increased non-selective cation whole-cell currents resulting in calcium increases that were fully blocked by either the TRPV1 antagonist capsazepine (CPZ) or removal of extracellular calcium. Similarly, heating from room temperature to over 40\u00a0\u00b0C increased the same currents resulting in calcium increases that were significantly reduced by the TRP channel blockers lanthanum chloride (La3+) (100\u00a0\u03bcM) and ruthenium-red (RuR) (10\u00a0\u03bcM), respectively. Moreover, application of the TRPV channel opener 2-aminoethoxydiphenyl borate (2-APB) (400\u00a0\u03bcM) led to a reversible increase in intracellular Ca<sup>2+<\/sup>\u00a0indicating putative TRPV1\u20133 channel activity. Taken together, TRPV activity modulation by temperature underlies essential homeostatic mechanisms contributing to the support of corneal endothelial function under different ambient conditions.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45],"resource_type":[75],"application_areas":[50],"therapeutic_areas":[],"target_categories":[337,338],"target_families":[339],"targets":[101,121,238],"resource_keyword":[],"class_list":["post-4817","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","resource_type-publication","application_areas-ion-channels","target_categories-ligand-gated-ion-channels","target_categories-other-ion-channels","target_families-trp-channels","targets-trpv1","targets-trpv3","targets-tpv2"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/4817","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=4817"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=4817"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=4817"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=4817"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=4817"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=4817"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=4817"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=4817"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=4817"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}