{"id":4771,"date":"2023-02-17T07:10:00","date_gmt":"2023-02-17T07:10:00","guid":{"rendered":"http:\/\/ionchanneltargets.com\/resource_library\/trpm8-trpm8-activation-by-menthol-and-eucalyptol-performed-on-nanion%c2%92s-patchliner\/"},"modified":"2023-02-17T07:10:00","modified_gmt":"2023-02-17T07:10:00","slug":"2009-inhibitory-effect-of-pb2-on-the-transport-cycle-of-the-na-k-atpase","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2009-inhibitory-effect-of-pb2-on-the-transport-cycle-of-the-na-k-atpase\/","title":{"rendered":"2009 &#8211; Inhibitory effect of Pb2+ on the transport cycle of the Na<sup>+<\/sup>\/K<sup>+<\/sup>-ATPase"},"content":{"rendered":"<p>The effect of Pb2+\u00a0on the transport cycle of the Na<sup>+<\/sup>\/K<sup>+<\/sup>-ATPase was characterized in detail at a molecular level by combining electrical and biochemical measurements. Electrical measurements were performed by adsorbing purified membrane fragments containing Na<sup>+<\/sup>\/K<sup>+<\/sup>-ATPase on a solid-supported membrane. Upon adsorption, the Na<sup>+<\/sup>\/K<sup>+<\/sup>-ATPase was activated by carrying out concentration jumps of different activating substrates, for example, Na<sup>+<\/sup>\u00a0and ATP. Charge movements following Na<sup>+<\/sup>\/K<sup>+<\/sup>-ATPase activation were measured in the presence of various Pb2+\u00a0concentrations to investigate the effect of Pb2+\u00a0on different ion translocating steps of the pump cycle. These charge measurements were then compared to biochemical measurements of ATPase activity in the presence of increasing Pb2+ concentration. Our results indicate that Pb2+\u00a0inhibits cycling of the enzyme, but it does not affect cytoplasmic Na<sup>+<\/sup>\u00a0binding and release of Na<sup>+<\/sup>\u00a0ions at the extracellular side at concentrations below 10 \u03bcM. To explain the inhibitory effect of Pb2+\u00a0on the Na<sup>+<\/sup>\/K<sup>+<\/sup>-ATPase, we propose that Pb2+\u00a0may interfere with the hydrolytic cleavage of the phosphorylated intermediate E2P, which occurs in the K<sup>+<\/sup>-related branch of the pump cycle.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[108],"therapeutic_areas":[],"target_categories":[60],"target_families":[344],"targets":[208],"resource_keyword":[],"class_list":["post-4771","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-transporters","target_categories-transporters","target_families-p-type-atpases","targets-na-k-atpase"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/4771","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=4771"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=4771"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=4771"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=4771"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=4771"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=4771"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=4771"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=4771"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=4771"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}