{"id":3249,"date":"2023-02-17T07:09:27","date_gmt":"2023-02-17T07:09:27","guid":{"rendered":"http:\/\/ionchanneltargets.com\/resource_library\/2022-conventional-rigid-2d-substrates-cause-complex-contractile-signals-in-monolayers-of-human-induced-pluripotent-stem-cell-derived-cardiomyocytes\/"},"modified":"2023-02-17T07:09:28","modified_gmt":"2023-02-17T07:09:28","slug":"1999-charge-translocation-by-the-na-k-atpase-investigated-on-solid-supported-membranes-cytoplasmic-cation-binding-and-release","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/1999-charge-translocation-by-the-na-k-atpase-investigated-on-solid-supported-membranes-cytoplasmic-cation-binding-and-release\/","title":{"rendered":"1999 &#8211; Charge Translocation by the Na<sup>+<\/sup>\/K<sup>+<\/sup>-ATPase Investigated on Solid Supported Membranes: Cytoplasmic Cation Binding and Release"},"content":{"rendered":"<p>In the preceding publication (Pintschovius and Fendler, 1999. Biophys. J. 76:000\u2013000) a new technique was described that was able to produce concentration jumps of arbitrary ion species at the surface of a solid supported membrane (SSM). This technique can be used to investigate the kinetics of ion translocating proteins adsorbed to the SSM. Charge translocation of the Na<sup>+<\/sup>\/K<sup>+<\/sup>-ATPase in the presence of ATP was investigated. Here we describe experiments carried out with membrane fragments containing Na<sup>+<\/sup>\/K<sup>+<\/sup>-ATPase from pig kidney and in the absence of ATP. Electrical currents are measured after rapid addition of Na<sup>+<\/sup>. We demonstrate that these currents can be explained only by a cation binding process on the cytoplasmic side, most probably to the cytoplasmic cation binding site of the Na<sup>+<\/sup>\/K<sup>+<\/sup>-ATPase. An electrogenic reaction of the protein was observed only with Na<sup>+<\/sup>, but not with other monovalent cations (K<sup>+<\/sup>, Li+, Rb+, Cs+). Using Na<sup>+<\/sup>\u00a0activation of the enzyme after preincubation with K<sup>+<\/sup>\u00a0we also investigated the K<sup>+<\/sup>-dependent half-cycle of the Na<sup>+<\/sup>\/K<sup>+<\/sup>-ATPase. A rate constant for K<sup>+<\/sup>\u00a0translocation in the absence of ATP of 0.2\u20130.3 s\u22121 was determined. In addition, these experiments show that K<sup>+<\/sup>\u00a0deocclusion, and cytoplasmic K<sup>+<\/sup>\u00a0release are electroneutral.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[108],"therapeutic_areas":[],"target_categories":[60],"target_families":[344],"targets":[208],"resource_keyword":[],"class_list":["post-3249","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-transporters","target_categories-transporters","target_families-p-type-atpases","targets-na-k-atpase"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/3249","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=3249"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=3249"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=3249"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=3249"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=3249"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=3249"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=3249"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=3249"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=3249"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}