{"id":16981,"date":"2025-03-04T10:59:53","date_gmt":"2025-03-04T10:59:53","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=16981"},"modified":"2025-03-04T10:59:53","modified_gmt":"2025-03-04T10:59:53","slug":"2025-structure-and-dynamics-of-a-multidomain-ligand-gated-ion-channel-revealed-under-acidic-conditions","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2025-structure-and-dynamics-of-a-multidomain-ligand-gated-ion-channel-revealed-under-acidic-conditions\/","title":{"rendered":"2025 &#8211; Structure and dynamics of a multidomain ligand-gated ion channel revealed under acidic conditions"},"content":{"rendered":"<p>Ligand-gated ion channels are critical mediators of electrochemical signal transduction across evolution. The bacterial channel DeCLIC constitutes a provocative model system for structure, function, and dynamics in this family, including a modulatory N-terminal domain (NTD). Previous closed structures of DeCLIC support a rationale for its inhibition by calcium; however, properties of its open state remain unclear. Here we used cryogenic electron microscopy under acidic conditions to determine a previously unreported conformation of DeCLIC with an expanded pore. This structure was relatively stable and permeable in simulations, and agreed with the average low-pH solution structure by small-angle neutron scattering. In the absence of calcium, an alternative closed class exhibited dynamic rearrangements in the NTD. We propose that our expanded-pore structure corresponds to a functional open state of DeCLIC, while calcium-site and NTD dynamics drive channel closure, providing a detailed template for modulatory mechanisms in the larger channel family.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[40],"resource_type":[75],"application_areas":[50,53],"therapeutic_areas":[143],"target_categories":[337],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-16981","resource_library","type-resource_library","status-publish","hentry","products_tags-orbit-mini","resource_type-publication","application_areas-ion-channels","application_areas-assay-development-and-validation","therapeutic_areas-assay-development-and-validation","target_categories-ligand-gated-ion-channels"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/16981","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=16981"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=16981"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=16981"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=16981"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=16981"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=16981"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=16981"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=16981"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=16981"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}