{"id":15763,"date":"2024-08-28T14:00:08","date_gmt":"2024-08-28T14:00:08","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=15763"},"modified":"2024-08-28T14:00:08","modified_gmt":"2024-08-28T14:00:08","slug":"2024-an-automated-planar-patch-clamp-approach-to-measure-the-membrane-potential-and-resting-membrane-currents-in-a-human-cerebrovascular-endothelial-cell-line","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2024-an-automated-planar-patch-clamp-approach-to-measure-the-membrane-potential-and-resting-membrane-currents-in-a-human-cerebrovascular-endothelial-cell-line\/","title":{"rendered":"2024 &#8211; An automated planar patch-clamp approach to measure the membrane potential and resting membrane currents in a human cerebrovascular endothelial cell line"},"content":{"rendered":"<div id=\"abs0010\">\n<p id=\"sect0010\" class=\"u-h4 u-margin-m-top u-margin-xs-bottom\">Background<\/p>\n<p id=\"sp0035\">The conventional \u201cwhole-cell patch-clamp\u201d recording technique is widely used to measure the resting membrane potential (V<sub>M<\/sub>) and to dissect the underlying membrane ionic conductances in isolated vascular endothelial cells.<\/p>\n<\/div>\n<div id=\"abs0015\">\n<p id=\"sect0015\" class=\"u-h4 u-margin-m-top u-margin-xs-bottom\">New method<\/p>\n<p id=\"sp0040\">Herein, we assessed whether the automated patch-clamp (APC) technology, which replaces the traditional patch-pipette with a planar substrate to permit researchers lacking formal training in electrophysiology to generate large amounts of data in a relatively short time, can be used to characterize the bioelectrical activity of vascular endothelial cells. We assessed whether the Port-a-Patch planar patch-clamp system, which is regarded as the smallest electrophysiological rig available on the market, can be used to measure the V<sub>M<\/sub>\u00a0and resting membrane currents in the human cerebrovascular endothelial cell line, hCMEC\/D3.<\/p>\n<\/div>\n<div id=\"abs0020\">\n<p id=\"sect0020\" class=\"u-h4 u-margin-m-top u-margin-xs-bottom\">Comparison with existing methods<\/p>\n<p id=\"sp0045\">We demonstrated that the Port-a-Patch planar patch-clamp system provides the same values of the resting V<sub>M<\/sub>\u00a0as those provided by the conventional patch-clamp technique. Furthermore, the APC technology provides preliminary data demonstrating that the resting V<sub>M<\/sub>\u00a0of hCMEC\/D3 cells is primarily contributed by Cl<sup>&#8211;<\/sup>\u00a0and Na<sup>+<\/sup>, as demonstrated with the patch-clamp technique for many other endothelial cell types.<\/p>\n<\/div>\n<div id=\"abs0025\">\n<p id=\"sect0025\" class=\"u-h4 u-margin-m-top u-margin-xs-bottom\">Conclusions<\/p>\n<p id=\"sp0050\">The Port-a-Patch planar patch-clamp system can be successfully used to measure the resting V<sub>M<\/sub>\u00a0and the underlying membrane ionic conductances in hCMEC\/D3 cells. We envisage that this easy-to-use APC system could also be extremely useful for the investigation of the membrane currents that can be activated by chemical, thermal, optical, and mechanical stimuli in this cell line as well as in other types of isolated vascular endothelial cells.<\/p>\n<\/div>\n","protected":false},"featured_media":0,"template":"","products_tags":[45],"resource_type":[75],"application_areas":[50,53],"therapeutic_areas":[143],"target_categories":[],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-15763","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","resource_type-publication","application_areas-ion-channels","application_areas-assay-development-and-validation","therapeutic_areas-assay-development-and-validation"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/15763","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=15763"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=15763"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=15763"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=15763"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=15763"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=15763"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=15763"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=15763"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=15763"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}