{"id":15762,"date":"2024-08-28T13:55:44","date_gmt":"2024-08-28T13:55:44","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=15762"},"modified":"2024-08-28T13:55:44","modified_gmt":"2024-08-28T13:55:44","slug":"2024-first-record-of-paralytic-shellfish-toxins-in-marine-pufferfish-from-the-spanish-mediterranean-coast-using-cell-based-assay-automated-patch-clamp-and-hplc-fld","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2024-first-record-of-paralytic-shellfish-toxins-in-marine-pufferfish-from-the-spanish-mediterranean-coast-using-cell-based-assay-automated-patch-clamp-and-hplc-fld\/","title":{"rendered":"2024 &#8211; First record of paralytic shellfish toxins in marine pufferfish from the Spanish Mediterranean coast using cell-based assay, automated patch clamp and HPLC-FLD"},"content":{"rendered":"<p>Pufferfish is one of the most poisonous marine organisms, responsible for numerous poisoning incidents and some human fatalities due to its capability to accumulate potent neurotoxins such as tetrodotoxins (TTXs) and paralytic shellfish toxins (PSTs). In this study, tissue extracts (muscle, skin, liver, intestinal tract and gonads) obtained from sixteen pufferfish specimens of the\u00a0<em>Lagocephalus lagocephalus<\/em>\u00a0and\u00a0<em>Sphoeroides pachygaster<\/em>\u00a0species, collected along the Spanish Mediterranean coast, were analysed for the presence of voltage-gated sodium channel (also known as Na<sub>v<\/sub>\u00a0channel) blockers using cell-based assay (CBA) and automated patch clamp (APC). No toxicity was observed in any of the\u00a0<em>S. pachygaster<\/em>\u00a0specimens, but toxicity was detected in the liver of most\u00a0<em>L. lagocephalus<\/em>\u00a0specimens. Instrumental analysis of these specimens, as well as in one\u00a0<em>Lagocephalus sceleratus<\/em>\u00a0specimen, by high-performance liquid chromatography coupled to fluorescence detection (HPLC-FLD) was performed, which confirmed the presence of PSTs only in\u00a0<em>L. lagocephalus<\/em>\u00a0specimens. This analysis reported the presence of saxitoxin (STX) and decarbamoylsaxitoxin (dcSTX) in all positive samples, being dcSTX the major analogue. These results demonstrate the ability of this species to accumulate PSTs, being the first report of the presence of PSTs in Mediterranean\u00a0<em>L.<\/em>\u00a0<em>lagocephalus<\/em>\u00a0specimens. Furthermore, the presence of high PSTs contents in all five tested tissues of one\u00a0<em>L. lagocephalus<\/em>\u00a0specimen pointed the risk that the presence of this toxic fish in the Mediterranean Sea may represent for seafood safety and human health in case of accidental consumption.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[44],"resource_type":[75],"application_areas":[50,53,76],"therapeutic_areas":[143,290],"target_categories":[61],"target_families":[332],"targets":[],"resource_keyword":[],"class_list":["post-15762","resource_library","type-resource_library","status-publish","hentry","products_tags-patchliner","resource_type-publication","application_areas-ion-channels","application_areas-assay-development-and-validation","application_areas-drug-discovery","therapeutic_areas-assay-development-and-validation","therapeutic_areas-toxicology","target_categories-voltage-gated-ion-channels","target_families-nav-channels"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/15762","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=15762"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=15762"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=15762"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=15762"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=15762"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=15762"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=15762"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=15762"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=15762"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}