{"id":13418,"date":"2023-05-17T08:29:11","date_gmt":"2023-05-17T08:29:11","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=13418"},"modified":"2024-01-17T08:30:06","modified_gmt":"2024-01-17T08:30:06","slug":"2023-g-quadruplex-filtered-selective-ion-to-ion-current-amplification-for-non-invasive-ion-monitoring-in-real-time","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2023-g-quadruplex-filtered-selective-ion-to-ion-current-amplification-for-non-invasive-ion-monitoring-in-real-time\/","title":{"rendered":"2023 &#8211; G-Quadruplex-Filtered Selective Ion-to-Ion Current Amplification for Non-Invasive Ion Monitoring in Real Time"},"content":{"rendered":"<p>Living cells efflux intracellular ions for maintaining cellular life, so intravital measurements of specific ion signals are of significant importance for studying cellular functions and pharmacokinetics. In this work, de novo synthesis of artificial K<sup>+<\/sup>-selective membrane and its integration with polyelectrolyte hydrogel-based open-junction ionic diode (OJID) is demonstrated, achieving a real-time K<sup>+<\/sup>-selective ion-to-ion current amplification in complex bioenvironments. By mimicking biological K<sup>+<\/sup>\u00a0channels and nerve impulse transmitters, in-line K<sup>+<\/sup>-binding G-quartets are introduced across freestanding lipid bilayers by G-specific hexylation of monolithic G-quadruplex, and the pre-filtered K<sup>+<\/sup>\u00a0flow is directly converted to amplified ionic currents by the OJID with a fast response time at 100\u00a0ms intervals. By the synergistic combination of charge repulsion, sieving, and ion recognition, the synthetic membrane allows K<sup>+<\/sup>\u00a0transport exclusively without water leakage; it is 250\u00d7 and 17\u00d7 more permeable toward K<sup>+<\/sup>\u00a0than monovalent anion, Cl<sup>\u2212<\/sup>, and polyatomic cation,\u00a0<i>N<\/i>-methyl-<span class=\"smallCaps\">d<\/span>-glucamine<sup>+<\/sup>, respectively. The molecular recognition-mediated ion channeling provides a 500% larger signal for K<sup>+<\/sup>\u00a0as compared to Li<sup>+<\/sup>\u00a0(0.6\u00d7 smaller than K<sup>+<\/sup>) despite the same valence. Using the miniaturized device, non-invasive, direct, and real-time K<sup>+<\/sup>\u00a0efflux monitoring from living cell spheroids is achieved with minimal crosstalk, specifically in identifying osmotic shock-induced necrosis and drug-antidote dynamics.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[40],"resource_type":[75],"application_areas":[53],"therapeutic_areas":[143],"target_categories":[],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-13418","resource_library","type-resource_library","status-publish","hentry","products_tags-orbit-mini","resource_type-publication","application_areas-assay-development-and-validation","therapeutic_areas-assay-development-and-validation"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/13418","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=13418"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=13418"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=13418"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=13418"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=13418"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=13418"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=13418"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=13418"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=13418"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}