{"id":13390,"date":"2024-01-11T11:09:03","date_gmt":"2024-01-11T11:09:03","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=13390"},"modified":"2024-01-11T11:09:25","modified_gmt":"2024-01-11T11:09:25","slug":"2023-functional-and-structural-asymmetry-suggest-a-unifying-principle-for-catalysis-in-membrane-bound-pyrophosphatases","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2023-functional-and-structural-asymmetry-suggest-a-unifying-principle-for-catalysis-in-membrane-bound-pyrophosphatases\/","title":{"rendered":"2024 &#8211; Functional and structural asymmetry suggest a unifying principle for catalysis in membrane-bound pyrophosphatases"},"content":{"rendered":"<p>Membrane-bound pyrophosphatases (M-PPases) are homodimeric primary ion pumps that couple the transport of Na<sup>+<\/sup>&#8211; and\/or H<sup>+<\/sup>\u00a0across membranes to the hydrolysis of pyrophosphate. Their role in the virulence of protist pathogens like\u00a0<i>Plasmodium falciparum<\/i>\u00a0makes them an intriguing target for structural and functional studies. Here, we show the first structure of a K<sup>+<\/sup>-independent M-PPase, asymmetric and time-dependent substrate binding in time-resolved structures of a K<sup>+<\/sup>-dependent M-PPase and demonstrate pumping-before-hydrolysis by electrometric studies. We suggest how key residues in helix 12, 13, and the exit channel loops affect ion selectivity and K<sup>+<\/sup>-activation due to a complex interplay of residues that are involved in subunit-subunit communication. Our findings not only explain ion selectivity in M-PPases but also why they display half-of-the-sites reactivity. Based on this, we propose, for the first time, a unified model for ion-pumping, hydrolysis, and energy coupling in\u00a0<i>all<\/i>\u00a0M-PPases, including those that pump both Na<sup>+<\/sup>\u00a0and H<sup>+<\/sup>.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[108],"therapeutic_areas":[],"target_categories":[60],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-13390","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-transporters","target_categories-transporters"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/13390","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=13390"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=13390"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=13390"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=13390"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=13390"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=13390"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=13390"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=13390"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=13390"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}