{"id":13232,"date":"2024-01-04T14:24:46","date_gmt":"2024-01-04T14:24:46","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=13232"},"modified":"2024-01-04T14:24:46","modified_gmt":"2024-01-04T14:24:46","slug":"2023-structure-and-function-of-a-yeee-yeed-complex-for-sophisticated-thiosulfate-uptake","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2023-structure-and-function-of-a-yeee-yeed-complex-for-sophisticated-thiosulfate-uptake\/","title":{"rendered":"2023 &#8211; Structure and function of a YeeE-YeeD complex for sophisticated thiosulfate uptake"},"content":{"rendered":"<p>Uptake of thiosulfate ions as an inorganic sulfur source from the environment is important for bacterial sulfur assimilation. Recently, a selective thiosulfate uptake pathway involving membrane protein YeeE (TsuA) was characterized. However, the precise function of YeeE and a putative cofactor in the<br \/>\nthiosulfate ion uptake pathway remained unclear. Here, we assessed selective thiosulfate transport via YeeE in vitro and characterized YeeD (TsuB) as an adjacent and essential cofactor for YeeE-mediated thiosulfate uptake in vivo. We further showed that YeeD possesses thiosulfate decomposition activity<br \/>\nand that a conserved cysteine in YeeD was modified in several forms in the presence of thiosulfate. Finally, the crystal structure of a YeeE-YeeD fusion protein at 2.6-\u00c5 resolution revealed their interactions. The association was evaluated by a binding assay using purified proteins. Based on these results, a model of the sophisticated uptake of thiosulfate ions by YeeE and YeeD is proposed.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[53,108],"therapeutic_areas":[143],"target_categories":[60],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-13232","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-assay-development-and-validation","application_areas-transporters","therapeutic_areas-assay-development-and-validation","target_categories-transporters"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/13232","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=13232"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=13232"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=13232"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=13232"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=13232"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=13232"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=13232"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=13232"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=13232"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}