{"id":12478,"date":"2023-09-01T11:16:55","date_gmt":"2023-09-01T11:16:55","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=12478"},"modified":"2023-09-01T11:16:55","modified_gmt":"2023-09-01T11:16:55","slug":"2023-cell-free-synthesis-and-electrophysiological-analysis-of-multipass-voltage-gated-ion-channels-tethered-in-microsomal-membranes","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2023-cell-free-synthesis-and-electrophysiological-analysis-of-multipass-voltage-gated-ion-channels-tethered-in-microsomal-membranes\/","title":{"rendered":"2023 &#8211; Cell-Free Synthesis and Electrophysiological Analysis of Multipass Voltage-Gated Ion Channels Tethered in Microsomal Membranes"},"content":{"rendered":"<p>Cell-free protein synthesis (CFPS) has emerged as a powerful tool for the rapid synthesis and analysis of various structurally and functionally distinct proteins. These include \u2018difficult-to-express\u2019 membrane proteins such as large multipass ion channel receptors. Owing to their membrane localization, eukaryotic CFPS supplemented with endoplasmic reticulum (ER)-derived microsomal vesicles has proven to be an efficient system for the synthesis of functional membrane proteins. Here we demonstrate the applicability of the eukaryotic cell-free systems based on lysates from the mammalian Chinese Hamster Ovary (CHO) and insect\u00a0<i>Spodoptera frugiperda<\/i>\u00a0(<i>Sf<\/i>21<i>)<\/i>\u00a0cells. We demonstrate the efficiency of the systems in the\u00a0<i>de novo<\/i>\u00a0cell-free synthesis of the human cardiac ion channels: ether-a-go-go potassium channel (hERG) K<sub>V<\/sub>11.1 and the voltage-gated sodium channel hNa<sub>V<\/sub>1.5.<\/p>\n<p>Read more in the publication <a href=\"https:\/\/link.springer.com\/chapter\/10.1007\/10_2023_228#DOI\">here<\/a>.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[98],"resource_type":[75],"application_areas":[50,53],"therapeutic_areas":[143],"target_categories":[61],"target_families":[332,333],"targets":[92,93],"resource_keyword":[],"class_list":["post-12478","resource_library","type-resource_library","status-publish","hentry","products_tags-orbit-16-tc","resource_type-publication","application_areas-ion-channels","application_areas-assay-development-and-validation","therapeutic_areas-assay-development-and-validation","target_categories-voltage-gated-ion-channels","target_families-nav-channels","target_families-kv-channels","targets-kv11-1-herg","targets-nav1-5"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/12478","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=12478"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=12478"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=12478"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=12478"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=12478"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=12478"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=12478"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=12478"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=12478"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}