{"id":11162,"date":"2023-05-10T15:21:30","date_gmt":"2023-05-10T15:21:30","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=resource_library&#038;p=11162"},"modified":"2024-01-11T12:14:42","modified_gmt":"2024-01-11T12:14:42","slug":"2023-a-bioelectrochemical-approach-based-on-a-solid-supported-membrane-to-evaluate-the-effect-of-natural-products-on-ca2-atpase-the-case-of-6-gingerol","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/zh-hans\/resource_library\/2023-a-bioelectrochemical-approach-based-on-a-solid-supported-membrane-to-evaluate-the-effect-of-natural-products-on-ca2-atpase-the-case-of-6-gingerol\/","title":{"rendered":"2023 &#8211; A bioelectrochemical approach based on a solid supported membrane to evaluate the effect of natural products on Ca2+-ATPase: The case of 6-gingerol"},"content":{"rendered":"<p>The sarco(endo)plasmic reticulum Ca<sup>2+<\/sup>-ATPase (SERCA) plays an essential role in maintaining the low cytosolic Ca<sup>2+<\/sup>\u00a0level that enables a variety of cellular processes. SERCA couples\u00a0ATP\u00a0hydrolysis to the transport of two Ca<sup>2+<\/sup>\u00a0ions against their electrochemical potential gradient from the cytoplasm into the lumen of the sarco\/endoplasmic reticulum (SR\/ER). Because of its central role in regulating cytoplasmic Ca<sup>2+<\/sup>\u00a0concentration, SERCA dysfunction has been associated with several pathological conditions. Stimulation of SERCA activity may represent a potential therapeutic strategy in various disease states connected with dysfunctional SERCA. The natural phenolic compound 6-gingerol, the most abundant and the major biologically active compound of ginger, was reported to activate the SERCA enzyme. The present study aimed at investigating the effect of 6-gingerol on SERCA transport activity using a bioelectrochemical approach based on a solid supported membrane (SSM). We first performed a voltammetric characterization of 6-gingerol to better understand its electrochemical behavior. We then studied the interaction of 6-gingerol with SR vesicles containing SERCA adsorbed on the SSM electrode. The measured current signals indicated that ATP-dependent Ca<sup>2+<\/sup>\u00a0translocation by SERCA was remarkably increased in the presence of 6-gingerol at low micromolar concentration. We also found that 6-gingerol has a rather high affinity for SERCA (EC<sub>50<\/sub>\u00a0of 1.8\u00a0\u00b1\u00a00.3\u00a0\u00b5M), and SERCA activation by 6-gingerol is reversible. The observed stimulatory effect of 6-gingerol on SERCA Ca<sup>2+<\/sup>-translocating activity may be beneficial in the prevention and\/or treatment of pathological conditions related to SERCA dysfunction.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[75],"application_areas":[108],"therapeutic_areas":[143],"target_categories":[60],"target_families":[344],"targets":[207],"resource_keyword":[],"class_list":["post-11162","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-publication","application_areas-transporters","therapeutic_areas-assay-development-and-validation","target_categories-transporters","target_families-p-type-atpases","targets-serca"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library\/11162","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=11162"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/products_tags?post=11162"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_type?post=11162"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/application_areas?post=11162"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/therapeutic_areas?post=11162"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_categories?post=11162"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/target_families?post=11162"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/targets?post=11162"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/resource_keyword?post=11162"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}