{"id":16117,"date":"2024-04-04T12:41:14","date_gmt":"2024-04-04T12:41:14","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=products&p=16117"},"modified":"2024-09-24T12:36:11","modified_gmt":"2024-09-24T12:36:11","slug":"how-it-works","status":"publish","type":"products","link":"https:\/\/nanion.com\/zh-hans\/products\/atlaz\/how-it-works\/","title":{"rendered":"How it works"},"content":{"rendered":"
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Quantitative live-cell analytics<\/h4><\/div><\/div><\/div><\/div>
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The real-time, label-free monitoring of cellular behavior, including adhesion, proliferation, migration, and response to external stimuli, provides valuable insights into various biological processes and drug effects. In cellular biology, an impedance-based assay can be used to measure those changes and effects in cells. To measure impedance, small electrical currents are delivered to electrodes embedded in the cell culture substrate. Initially, when no cells are present, the electrical current flows easily, resulting in low impedance. However, upon introducing cells onto the substrate, they obstruct these electrical currents, leading to an increase in impedance.<\/p>\n

This technique enables the creation of cell-specific profiles, aiding in the observation of various proliferation and cell behavior patterns. Impedance is also sensitive to subtle changes in cell conformation, such as those caused by receptor-mediated signaling. If a perturbation causes cells to die or detach, the impedance decreases.<\/p>\n

The AtlaZ device operates by assessing changes in the complex impedance of a cell monolayer, e.g. triggered by receptor stimulation, effector cells such as CAR-T cells or toxic compounds. The system provides quantitative live-cell analysis by analysing the Cell Signal (Impedance IZI in Ohm) of adherent cells grown on 96-well plates with embedded planar gold-film electrodes.<\/p>\n<\/div>\n<\/div><\/div>

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