{"id":16084,"date":"2023-02-12T12:05:19","date_gmt":"2023-02-12T12:05:19","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=products&p=16084"},"modified":"2024-09-24T12:18:22","modified_gmt":"2024-09-24T12:18:22","slug":"how-it-works","status":"publish","type":"products","link":"https:\/\/nanion.com\/zh-hans\/products\/surfe%c2%b2r-n1\/how-it-works\/","title":{"rendered":"How it works"},"content":{"rendered":"
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Measuring transporter currents<\/h4><\/div><\/div><\/div><\/div>
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Solid supported membrane (SSM)-based electrophysiology differs from conventional electrophysiology such as patch-clamp, since no living cells are required, but rather diverse native or artificial membrane vesicles. The samples used range from reconstituted protein in proteoliposomes to membrane preparations from organelles or plasma membrane. For the sample preparation bacterial cells, eukaryotic cell culture or native tissue can be used. The membrane sample is added to an SSM in advance of the experiment. This leads to the stable adsorption of the membranes to the SSM and the formation of a capacitively coupled compound membrane. The SSM itself consists of a lipid monolayer on top of a thiolated gold coated sensor chip. One important advantage compared to patch-clamp is the large sensor size of up to 3 mm. This allows the measurement of about 109<\/sup> transporters at the same time and yields a significant improvement of signal to noise ratio. Therefore low-turnover targets become accessible to electrophysiological characterization.<\/p>\n<\/div>\n<\/div><\/div>

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