{"id":15954,"date":"2024-08-13T13:46:46","date_gmt":"2024-08-13T13:46:46","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=news&#038;p=15954"},"modified":"2024-09-09T12:41:21","modified_gmt":"2024-09-09T12:41:21","slug":"advances-in-automated-patch-clamp-analyses-of-red-blood-cells","status":"publish","type":"news","link":"https:\/\/nanion.com\/zh-hans\/news\/advances-in-automated-patch-clamp-analyses-of-red-blood-cells\/","title":{"rendered":"Advances in automated patch clamp analyses of red blood cells"},"content":{"rendered":"<div id=\"pl-15581\" class=\"panel-layout\">\n<div id=\"pg-15581-0\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;full_height&quot;:&quot;&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;lsow_dark_bg&quot;:&quot;&quot;,&quot;bottom_margin&quot;:&quot;10px&quot;,&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div id=\"pgc-15581-0-0\" class=\"panel-grid-cell\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;vertical_alignment&quot;:&quot;auto&quot;}\" data-weight=\"1\">\n<div id=\"panel-15581-0-0-0\" class=\"so-panel widget widget_text panel-first-child\" data-index=\"0\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;font_bold&quot;:&quot;&quot;}\">\n<div class=\"textwidget\">\n<p><strong>When: <\/strong>September 19th, 2024 from 16:00 &#8211; 17:00 h CEST<\/p>\n<p><strong>Venue:<\/strong> via Zoom<\/p>\n<\/div><\/div>\n<div id=\"panel-15581-0-0-1\" class=\"so-panel widget widget_sow-button panel-last-child\" data-index=\"1\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;font_bold&quot;:&quot;&quot;}\"><div class=\"so-widget-sow-button so-widget-sow-button-flat-24c0153bf600\"><div class=\"ow-button-base ow-button-align-left\">\n\t\t\t<a href=\"\/webinar-registration-2\/\" class=\"btn-primary sf-form sowb-button ow-icon-placement-left ow-button-hover\" target=\"_blank\" rel=\"noopener noreferrer\">\n\t\t<span>\n\t\t\t\n\t\t\tRegister here\t\t<\/span>\n\t\t\t<\/a>\n\t<\/div>\n<\/div><\/div>\n<\/div>\n<\/div>\n<div id=\"pg-15581-1\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;full_height&quot;:&quot;&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;lsow_dark_bg&quot;:&quot;&quot;,&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div id=\"pgc-15581-1-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-15581-1-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child\" data-index=\"2\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;font_bold&quot;:&quot;&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<h4><\/h4>\n<p>&nbsp;<\/p>\n<h4><strong><span style=\"color: #000000;\">Summary of the webinar<\/span><\/strong><\/h4>\n<p>Explore advanced electrophysiological techniques in red blood cells. This webinar covers automated patch clamp for ion channel study, highlighting the roles of Piezo1, K<sub>Ca<\/sub>3.1, and TRPV2 in RBC functions and diseases, and the concept of Pseudo Action Potentials (PAPs) for understanding voltage-activated ion channels in RBCs.<\/p>\n<p>&nbsp;<\/p>\n<h3>Agenda:<\/h3>\n<p>&nbsp;<\/p>\n<ol>\n<li>Introduction\n<ul>\n<li><strong>Artem Kondratskyi<\/strong> (Scientific Solutions Manager, Nanion Technologies)<\/li>\n<\/ul>\n<\/li>\n<li>\u201cElectrophysiological characterization of red blood cells using the automated patch clamp\u201d\n<ul>\n<li><b>Nicoletta Murciano <\/b>(Application Scientist, Nanion Technologies)<\/li>\n<\/ul>\n<\/li>\n<li>\u201cPseudo Action Potentials (PAPs) in red blood cells \u2013&nbsp;an approach of understanding\u201d\n<ul>\n<li><strong>Lars Kaestner <\/strong>(Professor, Saarland University)<\/li>\n<\/ul>\n<\/li>\n<li>Joint discussion<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"panel-15581-1-0-1\" class=\"so-panel widget widget_sow-button panel-last-child\" data-index=\"3\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;font_bold&quot;:&quot;&quot;}\"><div class=\"so-widget-sow-button so-widget-sow-button-flat-24c0153bf600\"><div class=\"ow-button-base ow-button-align-left\">\n\t\t\t<a href=\"\/webinar-registration-2\/\" class=\"btn-primary sf-form sowb-button ow-icon-placement-left ow-button-hover\" target=\"_blank\" rel=\"noopener noreferrer\">\n\t\t<span>\n\t\t\t\n\t\t\tRegister here\t\t<\/span>\n\t\t\t<\/a>\n\t<\/div>\n<\/div><\/div>\n<\/div>\n<\/div>\n<div id=\"pg-15581-2\" class=\"panel-grid panel-has-style\" data-style=\"{&quot;padding&quot;:&quot;100px 0px -50px 0px&quot;,&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;full_height&quot;:&quot;&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;lsow_dark_bg&quot;:&quot;&quot;,&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div class=\"panel-row-style panel-row-style-for-15581-2\">\n<div id=\"pgc-15581-2-0\" class=\"panel-grid-cell\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;vertical_alignment&quot;:&quot;auto&quot;}\" data-weight=\"1\">\n<div id=\"panel-15581-2-0-0\" class=\"so-panel widget widget_media_image panel-first-child panel-last-child\" data-index=\"4\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;font_bold&quot;:&quot;&quot;}\"><a href=\"\/webinar-registration-2\/\" target=\"_blank\"><noscript><img decoding=\"async\" width=\"1625\" height=\"946\" src=\"https:\/\/nanion.com\/wp-content\/uploads\/2024\/08\/20240815_Webinar_Advances-in-automated-patch-clamp-analyses-of-red-blood-cells-V2_WEBSITE.png\" class=\"image wp-image-15633  attachment-full size-full\" alt style=\"max-width: 100%; height: auto;\" srcset=\"https:\/\/nanion.com\/wp-content\/uploads\/2024\/08\/20240815_Webinar_Advances-in-automated-patch-clamp-analyses-of-red-blood-cells-V2_WEBSITE.png 1625w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/08\/20240815_Webinar_Advances-in-automated-patch-clamp-analyses-of-red-blood-cells-V2_WEBSITE-300x175.png 300w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/08\/20240815_Webinar_Advances-in-automated-patch-clamp-analyses-of-red-blood-cells-V2_WEBSITE-1024x596.png 1024w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/08\/20240815_Webinar_Advances-in-automated-patch-clamp-analyses-of-red-blood-cells-V2_WEBSITE-768x447.png 768w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/08\/20240815_Webinar_Advances-in-automated-patch-clamp-analyses-of-red-blood-cells-V2_WEBSITE-1536x894.png 1536w\" sizes=\"(max-width: 1625px) 100vw, 1625px\"><\/noscript><img decoding=\"async\" width=\"1625\" height=\"946\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20viewBox%3D%220%200%201625%20946%22%3E%3C%2Fsvg%3E\" class=\"image wp-image-15633  attachment-full size-full lazyload\" alt style=\"max-width: 100%; height: auto;\" srcset=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20viewBox%3D%220%200%201625%20946%22%3E%3C%2Fsvg%3E 1625w\" sizes=\"(max-width: 1625px) 100vw, 1625px\" data-srcset=\"https:\/\/nanion.com\/wp-content\/uploads\/2024\/08\/20240815_Webinar_Advances-in-automated-patch-clamp-analyses-of-red-blood-cells-V2_WEBSITE.png 1625w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/08\/20240815_Webinar_Advances-in-automated-patch-clamp-analyses-of-red-blood-cells-V2_WEBSITE-300x175.png 300w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/08\/20240815_Webinar_Advances-in-automated-patch-clamp-analyses-of-red-blood-cells-V2_WEBSITE-1024x596.png 1024w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/08\/20240815_Webinar_Advances-in-automated-patch-clamp-analyses-of-red-blood-cells-V2_WEBSITE-768x447.png 768w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/08\/20240815_Webinar_Advances-in-automated-patch-clamp-analyses-of-red-blood-cells-V2_WEBSITE-1536x894.png 1536w\" data-src=\"https:\/\/nanion.com\/wp-content\/uploads\/2024\/08\/20240815_Webinar_Advances-in-automated-patch-clamp-analyses-of-red-blood-cells-V2_WEBSITE.png\"><\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"pg-15581-3\" class=\"panel-grid panel-no-style\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;full_height&quot;:&quot;&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;lsow_dark_bg&quot;:&quot;&quot;,&quot;cell_alignment&quot;:&quot;flex-start&quot;}\">\n<div id=\"pgc-15581-3-0\" class=\"panel-grid-cell\" data-weight=\"1\">\n<div id=\"panel-15581-3-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child\" data-index=\"5\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;margin&quot;:&quot;50px 0px 50px 0px&quot;,&quot;font_bold&quot;:&quot;&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<h3 class=\"widget-title\">Abstracts<\/h3>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<\/div>\n<\/div>\n<\/div>\n<div id=\"panel-15581-3-0-1\" class=\"so-panel widget widget_sow-editor\" data-index=\"6\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;font_bold&quot;:&quot;&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<h3 class=\"widget-title\">\n<h4><strong>Electrophysiological characterization of red blood cells using the automated patch clamp<\/strong><strong><\/strong><\/h4>\n<\/h3>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<p>Presented by Nicoletta Murciano (Application Scientist, Nanion Technologies)<\/p>\n<p>The ion channels Piezo1, K<sub>Ca<\/sub>3.1 (Gardos), and TRPV2 regulate Ca<sup>2+<\/sup> and control important functions of red blood cells (RBCs), such as volume adaptation and deformability. These functions are relevant during differentiation and in the circulation, while transporting gases throughout the body. In addition, dysfunctions of these channels can disrupt normal erythropoiesis and may be associated with diseases such as hereditary xerocytosis, Gardos channelopathy, sickle cell anaemia and thalassaemia, suggesting their potential as promising therapeutic targets.<\/p>\n<p>In this study, we examined the role of Ca<sup>2+<\/sup>-permeable and Ca<sup>2+<\/sup>-regulated channels in cultured erythroblasts (EBLs), cultured reticulocytes (retics), healthy and diseased RBCs using manual and high throughput automated patch clamp (APC, SyncroPatch 384). The latter allows for 384 parallel recordings of individual cells, thereby enabling the analysis of heterogeneous cell populations such as native RBC samples based on single cell responses.<\/p>\n<p>Our results have shown that a significantly higher percentage of EBLs and retics responded to Yoda1 compared to RBCs, indicating a higher activity of Piezo1 in the RBC progenitors, likely due to the different number of receptor copies during differentiation. Only in EBLs, Yoda1-induced current was significantly decreased in the presence of the specific Gardos blocker TRAM-34, suggesting that Gardos is mainly involved at early stages of differentiation. At later stages, calcium influx seemed insufficient to activate Gardos. Piezo1 was mechanically activated in EBLs, and not in retics or RBCs, possibly due to a different membrane tension and\/or increased expression of Piezo1 channel in EBLs. &nbsp;PIEZO1 was also analyzed in a patient with unknown mutations on PIEZO1 and spectrin, comparing its&nbsp; properties with those of healthy donors, revealing lower PIEZO1 activity in the patient. Manual patch clamp was used to investigate TRPV2 in RBCs of Sickle cell Anemia (SCA) patients by <sup>\u22069<\/sup>THC. SCA patient RBCs were more responsive to acute <sup>\u22069<\/sup>THC stimulation compared to control RBCs. These findings may help to interpret recent and very encouraging results on the use of cannabinoid derivatives (CBD) for the treatment of pain associated with the disease.<\/p>\n<p>This work provides valuable insights into the role of Piezo1, Gardos and TRPV2 channels in RBCs differentiation and diseases, and demonstrates that automated patch clamping provides robust assays to investigate Ca<sup>2+<\/sup>-regulating ion channels in primary cells. The high throughput technology overcomes challenges such as heterogeneous and\/or low expression of ion channels across a cell population. This makes the approach suited to detect channelopathies and to screen against diseases related to ion channel dysfunctions in general.<\/p>\n<hr>\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"panel-15581-3-0-2\" class=\"so-panel widget widget_sow-editor panel-last-child\" data-index=\"7\" data-style=\"{&quot;background_image_attachment&quot;:false,&quot;background_display&quot;:&quot;tile&quot;,&quot;background_image_size&quot;:&quot;full&quot;,&quot;background_image_opacity&quot;:&quot;100&quot;,&quot;border_thickness&quot;:&quot;1px&quot;,&quot;cvw_duration&quot;:&quot;300&quot;,&quot;cvw_easing&quot;:&quot;linear&quot;,&quot;cvw_anchor&quot;:&quot;top-bottom&quot;,&quot;cvw_repeat&quot;:&quot;true&quot;,&quot;font_bold&quot;:&quot;&quot;}\">\n<div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<h3 class=\"widget-title\">\n<h4><strong>Pseudo Action Potentials (PAPs) in red blood cells \u2013 an approach of understanding<\/strong><strong><\/strong><\/h4>\n<\/h3>\n<div class=\"siteorigin-widget-tinymce textwidget\">\n<p>Presented by Lars Kaestner (Professor, Saarland University)<\/p>\n<p>No cell could be more of a prototype of a non-excitable cell as red blood cells (RBCs). Nevertheless, there are numerous reports about voltage-activated ion channels in the membrane of red blood cells, e.g. How does it make sense? Are the ion channel detections just artefacts? Are they remnants left in red blood cells? Or is there a real physiological (or pathophysiological) function?<\/p>\n<p>The key methods applied are membrane potential measurements based on the Macey-Bennekou-Eg\u00e9e (MBE) method and patch-clamp. These measurements are complemented with [Ca<sup>2+<\/sup>]-imaging data of healthy human RBCs compared to RBCs from patients with a G\u00e1rdos-Channelopathy, with mouse RBCs from wild type and transgenic animals. Experiments are performed under various pharmacological stimulations including G\u00e1rdos channel activators and inhibitors NS309 and TRAM34, respectively, and the Ca<sub>V<\/sub>2.1 channel inhibitor omega-AgatoxinTK.<\/p>\n<p>In RBCs, activation of the G\u00e1rdos channel leads to cellular hyperpolarization (membrane potential drops from -12 mV to approximately -70 mV). Because of the low number of G\u00e1rdos channels per cell and its stochastic behavior (continuous openings and closings) there are conditions, when the membrane potential of RBCs flickers, i.e., jumping between -12 mV and&nbsp;-70 mV back and forward. These jumps are called Pseudo Action Potentials (PAPs) and our data support the notion that PAPs are able to induce voltage-activated Ca<sup>2+<\/sup>-entry, mediated by, e.g., Ca<sub>V<\/sub>2.1 channels.<\/p>\n<p>The induction of PAPs presents a feedback loop that enables the amplification of Ca<sup>2+<\/sup> signals. Ca<sup>2+<\/sup> signaling in RBCs is a highly complex process with multiple targets, including the (sustained) opening of the G\u00e1rdos channel, which is crucial for RBC volume adaptation. In this context, our data suggest the occurrence of PAPs in patients with G\u00e1rdos channelopathy.<\/p>\n<hr>\n<p>&nbsp;<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>When: September 19th, 2024 from 16:00 &#8211; 17:00 h C [&hellip;]<\/p>\n","protected":false},"featured_media":15634,"template":"","news_categories":[393],"class_list":["post-15954","news","type-news","status-publish","has-post-thumbnail","hentry","news_categories-event-zh-hans"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/news\/15954","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/news"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/news"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media\/15634"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=15954"}],"wp:term":[{"taxonomy":"news_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/news_categories?post=15954"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}