{"id":14267,"date":"2024-04-30T09:48:09","date_gmt":"2024-04-30T09:48:09","guid":{"rendered":"https:\/\/www.nanion.de\/?post_type=news&#038;p=14267"},"modified":"2025-01-02T14:50:47","modified_gmt":"2025-01-02T14:50:47","slug":"transporter-insights-from-shredding-serca-to-cell-free-synthesis-of-transporters","status":"publish","type":"news","link":"https:\/\/nanion.com\/zh-hans\/news\/transporter-insights-from-shredding-serca-to-cell-free-synthesis-of-transporters\/","title":{"rendered":"Transporter Ins]i[ghts: from shredding SERCA to cell-free synthesis of transporters"},"content":{"rendered":"<div id=\"pl-14267\" class=\"panel-layout\"><div id=\"pg-14267-0\" class=\"panel-grid panel-no-style\"><div id=\"pgc-14267-0-0\" class=\"panel-grid-cell\"><div id=\"panel-14267-0-0-0\" class=\"so-panel widget widget_text panel-first-child\" data-index=\"0\">\t\t\t<div class=\"textwidget\"><h4>Available on-demand. Watch now!<\/h4>\n<\/div>\n\t\t<\/div><div id=\"panel-14267-0-0-1\" class=\"so-panel widget widget_vido-lightbox-theme-widget\" data-index=\"1\"><div class=\"so-widget-vido-lightbox-theme-widget so-widget-vido-lightbox-theme-widget-default-d75171398898-14267\"><div class=\"video-lightbox\">\n                <div class=\"image\">\n                <div class=\"play-button\"><a href=\"https:\/\/www.youtube.com\/watch?v=2kEtSwwvNvc\" data-fancybox data-scrolling=\"no\"><\/a><\/div>\n                <noscript><img decoding=\"async\" width=\"3250\" height=\"1892\" src=\"https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue.png\" class=\"attachment-full size-full\" alt srcset=\"https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue.png 3250w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-300x175.png 300w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-1024x596.png 1024w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-768x447.png 768w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-1536x894.png 1536w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-2048x1192.png 2048w\" sizes=\"(max-width: 3250px) 100vw, 3250px\"><\/noscript><img decoding=\"async\" width=\"3250\" height=\"1892\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20viewBox%3D%220%200%203250%201892%22%3E%3C%2Fsvg%3E\" class=\"attachment-full size-full lazyload\" alt srcset=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20viewBox%3D%220%200%203250%201892%22%3E%3C%2Fsvg%3E 3250w\" sizes=\"(max-width: 3250px) 100vw, 3250px\" data-srcset=\"https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue.png 3250w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-300x175.png 300w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-1024x596.png 1024w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-768x447.png 768w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-1536x894.png 1536w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-2048x1192.png 2048w\" data-src=\"https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue.png\">            <\/div>\n        <\/div><\/div><\/div><div id=\"panel-14267-0-0-2\" class=\"so-panel widget widget_text\" data-index=\"2\">\t\t\t<div class=\"textwidget\"><p>&nbsp;<\/p>\n<p><strong>When:<\/strong> 23.05.2024, 16:00 \u2013 17:00 h CEST (8:00 &#8211; 9:00 h MDT)<\/p>\n<p><strong>Venue<\/strong>: via Zoom<\/p>\n<\/div>\n\t\t<\/div><div id=\"panel-14267-0-0-3\" class=\"so-panel widget widget_sow-button panel-last-child\" data-index=\"3\"><div class=\"so-widget-sow-button so-widget-sow-button-flat-24c0153bf600-14267\"><div class=\"ow-button-base ow-button-align-left\">\n\t\t\t<a class=\"btn-primary sf-form sowb-button ow-icon-placement-left ow-button-hover\">\n\t\t<span>\n\t\t\t\n\t\t\tRegister here\t\t<\/span>\n\t\t\t<\/a>\n\t<\/div>\n<\/div><\/div><\/div><\/div><div id=\"pg-14267-1\" class=\"panel-grid panel-no-style\"><div id=\"pgc-14267-1-0\" class=\"panel-grid-cell panel-grid-cell-empty\"><\/div><\/div><div id=\"pg-14267-2\" class=\"panel-grid panel-no-style\"><div id=\"pgc-14267-2-0\" class=\"panel-grid-cell\"><div id=\"panel-14267-2-0-0\" class=\"so-panel widget widget_sow-editor panel-first-child\" data-index=\"4\"><div class=\"so-widget-sow-editor so-widget-sow-editor-base\">\n<div class=\"siteorigin-widget-tinymce textwidget\">\n\t<hr>\n<h4><span style=\"color: #000000;\"><strong>Transporter Ins]i[ghts&nbsp;<\/strong><\/span><span style=\"color: #000000;\"><strong>Webinar<\/strong><\/span><span style=\"color: #000000;\"><strong>: from shredding SERCA to cell-free synthesis of transporters<\/strong><\/span><\/h4>\n<p>&nbsp;<\/p>\n<p>Join us for a fascinating webinar where Pawel Lojko (Technical University of Denmark, Irina Borodina\u00b4s lab), and Joseph Primeau (University of Alberta, Howard Young\u00b4s lab) will present their groundbreaking projects.<\/p>\n<p><strong>Irina Borodina\u2019s Group<\/strong>&nbsp;has developed an innovative workflow to overcome the challenges in characterizing membrane transporters. By integrating&nbsp;cell-free protein synthesis&nbsp;with&nbsp;solid supported membrane-based electrophysiology, they have crafted a cost-effective and technically efficient method. Utilizing&nbsp;nanodiscs&nbsp;for membrane protein synthesis within a&nbsp;continuous exchange cell-free system, they successfully expressed transporters that were then embedded into&nbsp;proteoliposomes. These were assayed for substrate specificity using the&nbsp;SURFE<sup>2<\/sup>R N1 instrument, paving the way for advanced transporter assays.<\/p>\n<p><strong>Howard Young\u2019s Group<\/strong>&nbsp;delves into the intricate molecular structure of&nbsp;Ca<sup>2+<\/sup> -ATPases&nbsp;from the&nbsp;endoplasmic reticulum (ER)&nbsp;and&nbsp;sarcoplasmic reticulum (SR). Their research aims to elucidate the molecular underpinnings of calcium transport and its regulatory mechanisms. These ATPases are vital for pumping calcium out of the cytosol, a process crucial for physiological functions such as heart muscle relaxation. Understanding these mechanisms is key, especially since defects in calcium transport are linked to various heart diseases, including&nbsp;hypertrophy, cardiomyopathy, hypertension, and&nbsp;end-stage heart failure.<\/p>\n<p>&nbsp;<\/p>\n<hr>\n<h4><\/h4>\n<h4><strong>Webinar Agenda:<\/strong><\/h4>\n<ol>\n<li>Introduction\n<ul>\n<li><strong>Andre Bazzone<\/strong> (Nanion Technologies)<\/li>\n<\/ul>\n<\/li>\n<li>\u201cCharacterizing Transporter Function Using Continuous-Exchange Cell-Free Synthesis and SSME\u201d\n<ul>\n<li><strong>Pawel Lojko<\/strong> (Technical University of Denmark, Irina Borodina\u00b4s lab)<\/li>\n<\/ul>\n<\/li>\n<li>\"Sarcolipin\u2019s role in regulating SERCA substrate-dependent calcium transport: <em>Shredding with the SURFE<sup>2<\/sup>R<\/em>\u201d\n<ul>\n<li><strong>Joseph Primeau<\/strong> (University of Alberta, Howard Young\u00b4s lab)<\/li>\n<\/ul>\n<\/li>\n<li>Joint Q&amp;A session<\/li>\n<\/ol>\n<\/div>\n<\/div><\/div><div id=\"panel-14267-2-0-1\" class=\"so-panel widget widget_sow-button panel-last-child\" data-index=\"5\"><div class=\"so-widget-sow-button so-widget-sow-button-flat-24c0153bf600-14267\"><div class=\"ow-button-base ow-button-align-left\">\n\t\t\t<a class=\"btn-primary sf-form sowb-button ow-icon-placement-left ow-button-hover\">\n\t\t<span>\n\t\t\t\n\t\t\tRegister here\t\t<\/span>\n\t\t\t<\/a>\n\t<\/div>\n<\/div><\/div><\/div><\/div><div id=\"pg-14267-3\" class=\"panel-grid panel-no-style\"><div id=\"pgc-14267-3-0\" class=\"panel-grid-cell\"><div id=\"panel-14267-3-0-0\" class=\"so-panel widget widget_media_image panel-first-child panel-last-child\" data-index=\"6\"><noscript><img decoding=\"async\" width=\"3250\" height=\"1892\" src=\"https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue.png\" class=\"image wp-image-14277  attachment-full size-full\" alt style=\"max-width: 100%; height: auto;\" srcset=\"https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue.png 3250w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-300x175.png 300w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-1024x596.png 1024w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-768x447.png 768w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-1536x894.png 1536w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-2048x1192.png 2048w\" sizes=\"(max-width: 3250px) 100vw, 3250px\"><\/noscript><img decoding=\"async\" width=\"3250\" height=\"1892\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20viewBox%3D%220%200%203250%201892%22%3E%3C%2Fsvg%3E\" class=\"image wp-image-14277  attachment-full size-full lazyload\" alt style=\"max-width: 100%; height: auto;\" srcset=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20viewBox%3D%220%200%203250%201892%22%3E%3C%2Fsvg%3E 3250w\" sizes=\"(max-width: 3250px) 100vw, 3250px\" data-srcset=\"https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue.png 3250w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-300x175.png 300w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-1024x596.png 1024w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-768x447.png 768w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-1536x894.png 1536w, https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue-2048x1192.png 2048w\" data-src=\"https:\/\/nanion.com\/wp-content\/uploads\/2024\/04\/20240523_Webinar-Transporter-insights_thumbnail-website-blue.png\"><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"<p>Available on-demand. Watch now! &nbsp; When: 23.05.2024 [&hellip;]<\/p>\n","protected":false},"featured_media":14277,"template":"","news_categories":[528,13],"class_list":["post-14267","news","type-news","status-publish","has-post-thumbnail","hentry","news_categories-front-page-news","news_categories-event"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/news\/14267","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/news"}],"about":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/types\/news"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media\/14277"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=14267"}],"wp:term":[{"taxonomy":"news_categories","embeddable":true,"href":"https:\/\/nanion.com\/zh-hans\/wp-json\/wp\/v2\/news_categories?post=14267"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}