{"id":7151,"date":"2023-02-17T07:00:22","date_gmt":"2023-02-17T07:00:22","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/14-07-2022-webinar-an-in-silico-in-vitro-pipeline-for-drug-cardiotoxicity-screening-identifies-ionic-proarrhythmia-mechanisms\/"},"modified":"2023-02-17T07:00:23","modified_gmt":"2023-02-17T07:00:23","slug":"14-07-2022-webinar-an-in-silico-in-vitro-pipeline-for-drug-cardiotoxicity-screening-identifies-ionic-proarrhythmia-mechanisms","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/14-07-2022-webinar-an-in-silico-in-vitro-pipeline-for-drug-cardiotoxicity-screening-identifies-ionic-proarrhythmia-mechanisms\/","title":{"rendered":"14.07.2022 | Webinar: An in silico-in vitro pipeline for drug cardiotoxicity screening identifies ionic proarrhythmia mechanisms"},"content":{"rendered":"<p>All new drugs are screened for their proarrhythmic potential using a method that is overly conservative and provides limited mechanistic insight, which can lead to the misclassification of beneficial drugs as proarrhythmic. Here, we developed an in silico-in vitro pipeline to circumvent these shortcomings. An iPSC-CM computational model was used to design electrophysiological voltage-clamp (VC) protocols for use during in vitro drug studies. Such VC data, along with AP recordings, were acquired from iPSC-CMs before and after treatment with a control solution or verapamil, cisapride, quinine, or quinidine. AP prolongation was seen in response to quinidine and quinine. The VC protocol identified all strong I<sup>Kr<\/sup> blockers. The protocol also detected block of ICaL by verapamil and Ito by quinidine. The VC data also uncovered a previously unidentified If block by quinine, which was confirmed with experiments using a HEK-293 expression system and automated patch-clamp.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[44],"resource_type":[320],"application_areas":[],"therapeutic_areas":[],"target_categories":[],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-7151","resource_library","type-resource_library","status-publish","hentry","products_tags-patchliner","resource_type-webinar"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/7151","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=7151"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=7151"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=7151"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=7151"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=7151"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=7151"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=7151"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=7151"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=7151"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}