{"id":7117,"date":"2023-02-17T06:51:03","date_gmt":"2023-02-17T06:51:03","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/13-10-2020-webinar-impedance-measurements-and-calcium-imaging-to-investigate-the-role-of-store-operated-ca2-entry-in-gpcr-mediated-endothelial-barrier-regulation\/"},"modified":"2023-02-17T06:51:04","modified_gmt":"2023-02-17T06:51:04","slug":"13-10-2020-webinar-impedance-measurements-and-calcium-imaging-to-investigate-the-role-of-store-operated-ca2-entry-in-gpcr-mediated-endothelial-barrier-regulation","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/13-10-2020-webinar-impedance-measurements-and-calcium-imaging-to-investigate-the-role-of-store-operated-ca2-entry-in-gpcr-mediated-endothelial-barrier-regulation\/","title":{"rendered":"13.10.2020 | Webinar: Impedance measurements and Calcium imaging to investigate the role of store operated Ca<sup>2+<\/sup> entry in GPCR-mediated endothelial barrier regulation"},"content":{"rendered":"<p>The endothelium of our blood vessels is under the control of various G protein-coupled receptors (GPCRs), regulating endothelial barrier function, vascular tone, angiogenesis and inflammation. Some of these GPCRs signal via Gq,11 protein, which activates Ca<sup>2+<\/sup>-release from the IP3-sensitive internal stores of the endoplasmic reticulum (ER). Calcium store depletion can subsequently activate so-called store-operated Ca<sup>2+<\/sup> entry (SOCE) via the ER-resident Ca<sup>2+<\/sup>-sensor stromal-interacting molecule 1 (STIM1) and Orai1 Ca<sup>2+<\/sup> entry channels.<\/p>\n<p>We used Impedance measurements and ratiometric Calcium imaging to investigate the role of calcium entry via Orai1 channels downstream of barrier-regulating GPCRs in response to their agonists Thrombin, Histamine and Sphingosine-1-Phosphate (S1P) in human endothelial cells.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[34],"resource_type":[320],"application_areas":[53,76,77],"therapeutic_areas":[],"target_categories":[],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-7117","resource_library","type-resource_library","status-publish","hentry","products_tags-cardioexcyte-96","resource_type-webinar","application_areas-assay-development-and-validation","application_areas-drug-discovery","application_areas-cell-ipscs-profiling"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/7117","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=7117"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=7117"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=7117"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=7117"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=7117"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=7117"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=7117"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=7117"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=7117"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}