{"id":7106,"date":"2023-02-17T07:12:29","date_gmt":"2023-02-17T07:12:29","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/2019-surfe2r-as-a-service-closing-the-gap-between-technology-and-application\/"},"modified":"2023-02-17T07:12:30","modified_gmt":"2023-02-17T07:12:30","slug":"2019-surfe2r-as-a-service-closing-the-gap-between-technology-and-application","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/2019-surfe2r-as-a-service-closing-the-gap-between-technology-and-application\/","title":{"rendered":"2019 &#8211; SURFE<sup>2<\/sup>R as a service &#8211; closing the gap between technology and application"},"content":{"rendered":"<p>Solid-supported membrane (SSM)-based electrophysiology allows functional characterization of transporters which are otherwise difficult to investigate due to their slow transport conductivity or intracellular localization. The &#8220;SURFE<sup>2<\/sup>R 96SE&#8221; platform makes this technology suitable for compound characterization at higher throughputs.<br \/>\nFor this case study, we prepared membrane vesicles from a PepT1-expressing cell line and screened an ion-channel\/transporter-focused library of 237 bioactive compounds for substrate and inhibitory activity. With the SURFE<sup>2<\/sup>R 96SE, the screen was completed in less than 8 hours with a success rate of 98%, demonstrating utility of the technology for compound screening.<br \/>\nOn the downside, application of SSM-based electrophysiology is limited to targets with an electrogenic net transport, assay setup is rather complex, and cost of ownership is significant. This may be an obstacle for implementing SURFE<sup>2<\/sup>R technology in-house. In response, Assay.Works entered into a partnership with Nanion, providing SURFE<sup>2<\/sup>R assays as a service. Backed by Nanions extensive expertise, we utilize our assay development and screening capabilities, including cell and membrane production, compound libraries, and sample management, to help clients realizing proof-of-concept, compound characterization, or profiling studies.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[132],"resource_type":[320],"application_areas":[53,76,108],"therapeutic_areas":[],"target_categories":[60],"target_families":[375],"targets":[136],"resource_keyword":[],"class_list":["post-7106","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-96se","resource_type-webinar","application_areas-assay-development-and-validation","application_areas-drug-discovery","application_areas-transporters","target_categories-transporters","target_families-symporter","targets-pept1-slc15a1"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/7106","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=7106"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=7106"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=7106"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=7106"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=7106"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=7106"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=7106"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=7106"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=7106"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}