{"id":6876,"date":"2023-02-17T07:18:45","date_gmt":"2023-02-17T07:18:45","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/neurons-electrophysiological-recordings-of-lgic-and-aa-transporters-in-icell-glutaneurons\/"},"modified":"2023-02-17T07:18:47","modified_gmt":"2023-02-17T07:18:47","slug":"neurons-electrophysiological-recordings-of-lgic-and-aa-transporters-in-icell-glutaneurons","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/neurons-electrophysiological-recordings-of-lgic-and-aa-transporters-in-icell-glutaneurons\/","title":{"rendered":"Neurons &#8211; &#8220;Electrophysiological recordings of LGIC and AA transporters in iCell<sup>\u00ae<\/sup> GlutaNeurons&#8221;"},"content":{"rendered":"<p>Human neurons derived from induced pluripotent stem cells (hiPSCs) are becoming increasingly important for studying basic neuronal physiology and can provide good models for studying neurological disorders. hiPSC derived neurons provide a viable alternative to primary cells and animal models in the drug discovery industry for finding novel therapeutics to treat seizure-related and neurodegenerative disorders. iCell<sup>\u00ae<\/sup>\u00a0GlutaNeurons are glutamatergic-enriched cortical neurons derived from hiPSCs. Single cell gene transcription analysis has shown the presence of glutamate receptors: AMPA, kainate and NMDA, as well as glutamate and GABA transporters. Ionotropic glutamate receptors mediate the majority of excitatory neurotransmission in the mammalian CNS and removal of glutamate from the synaptic cleft by reuptake via glutamate transporters plays a role in regulating neuronal excitability. GABA is the major inhibitory neurotransmitter in the brain and is important in controlling excitability. After release, GABA is removed from the extracellular space by GABA transporters(GATs), thus terminating inhibitory synaptic transmission. Both GABA and glutamate transporters may provide novel therapeutic targets for, e.g. Parkinson\u2019s disease, Alzheimer\u2019s disease, and epilepsy.We recorded ligand-gated ion channel currents mediated by GABAA\u00a0and AMPA receptors from iCell<sup>\u00ae<\/sup>\u00a0GlutaNeurons on the Patchliner and SyncroPatch 384PE. Furthermore, we could measure GABA and glutamate transporters in these neurons using the SURFE2R N1 device.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[42],"resource_type":[90],"application_areas":[50,108],"therapeutic_areas":[55],"target_categories":[337,60],"target_families":[355,375],"targets":[104,133,144],"resource_keyword":[],"class_list":["post-6876","resource_library","type-resource_library","status-publish","hentry","products_tags-syncropatch-384","resource_type-application-note","application_areas-ion-channels","application_areas-transporters","therapeutic_areas-cns","target_categories-ligand-gated-ion-channels","target_categories-transporters","target_families-ionotropic-glutamate-receptors","target_families-symporter","targets-glua2","targets-eaat3-slc1a1","targets-glun2a"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6876","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6876"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6876"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6876"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6876"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6876"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6876"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6876"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6876"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6876"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}