{"id":6866,"date":"2023-02-17T07:18:34","date_gmt":"2023-02-17T07:18:34","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/nachr%ce%b14%ce%b22-pharmacology-of-human-%ce%b14%ce%b22-nachr-recorded-on-the-syncropatch-384pe\/"},"modified":"2023-02-17T07:18:34","modified_gmt":"2023-02-17T07:18:34","slug":"nachr%ce%b14%ce%b22-pharmacology-of-human-%ce%b14%ce%b22-nachr-recorded-on-the-syncropatch-384pe","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/nachr%ce%b14%ce%b22-pharmacology-of-human-%ce%b14%ce%b22-nachr-recorded-on-the-syncropatch-384pe\/","title":{"rendered":"nAChR\u03b14\u03b22 &#8211; &#8220;Pharmacology of human \u03b14\u03b22 nAChR recorded on the SyncroPatch 384PE&#8221;"},"content":{"rendered":"<p>Nicotinic Acetylcholine Receptors (nAChR) are cationpermeable\u00a0ion channels, which mediate fast synaptic\u00a0transmission when activated by the endogenous\u00a0neurotransmitter acetylcholine (ACh) and the exogenous\u00a0natural alkaloid, nicotine. Neuronal nAChR form\u00a0pentameric channels which are composed of two \u03b1 (\u03b12\u00a0to \u03b110) and three \u03b2 subunits (\u03b22 to \u03b24). Mutations of\u00a0nAChR are associated with some forms of epilepsy and\u00a0many other neurological disorders such as Alzheimer\u2019s\u00a0Disease, Parkinson\u2019s, Tourette\u2019s Syndrome, Schizophrenia\u00a0and depression.\u00a0The most abundantly expressed nAChR in the mammalian\u00a0brain are the \u03b17 homomeric and \u03b14\u03b22 heteromeric\u00a0receptors. In contrast to the \u03b17, a4\u03b22 nAChR has a\u00a0high affinity for nicotine. This property, the up-regulation\u00a0during chronic exposure to nicotine, and the receptor\u00a0expression location in addiction sensitive regions of the\u00a0brain like the ventral tegmental area, strongly indicate\u00a0that the a4\u03b22 nAChR is a potential target for addiction\u00a0to nicotine.\u00a0Here we present data collected on the SyncroPatch 384PE showing activation and block of\u00a0\u03b14\u03b22 nAChR currents expressed in HEK cells with\u00a0rapid application of ligand (\u2018Ligand Puff\u2019). ACh\u00a0activates \u03b14\u03b22 nAChR in a concentration dependent\u00a0manner with an EC<sub>50<\/sub>\u00a0value similar to those\u00a0reported in the literature. Reproducible currents\u00a0were achieved when cells were preincubated with acetylcholinesterase (AChE). Finally, \u03b14\u03b22 nAChR were\u00a0blocked by dihydro-b-erythroidine hydrobromide (DH\u00dfE),\u00a0a well known competitive antagonist of the \u03b14 subunit3\u00a0with an IC<sub>50<\/sub>\u00a0in good agreement with the literature.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[42],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[55],"target_categories":[337],"target_families":[360],"targets":[194],"resource_keyword":[],"class_list":["post-6866","resource_library","type-resource_library","status-publish","hentry","products_tags-syncropatch-384","resource_type-application-note","application_areas-ion-channels","therapeutic_areas-cns","target_categories-ligand-gated-ion-channels","target_families-nicotinic-acetylcholine-receptors","targets-achr-4-2"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6866","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6866"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6866"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6866"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6866"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6866"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6866"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6866"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6866"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6866"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}