{"id":6856,"date":"2023-02-17T07:18:14","date_gmt":"2023-02-17T07:18:14","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/hclc-1-characterization-of-hclc-1-on-the-syncropatch-384\/"},"modified":"2023-02-17T07:18:16","modified_gmt":"2023-02-17T07:18:16","slug":"hclc-1-characterization-of-hclc-1-on-the-syncropatch-384","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/hclc-1-characterization-of-hclc-1-on-the-syncropatch-384\/","title":{"rendered":"hClC-1 &#8211; &#8220;Characterization of hClC-1 on the SyncroPatch 384&#8221;"},"content":{"rendered":"<p>The voltage-gated chloride channel gene (ClC) family is highly conserved and their members are present in both pro- and eukaryotes. In mammals, nine different ClC subtypes have been identified, which differ according\u00a0to tissue distribution and subcellular location. ClC-1 is exclusively expressed in skeletal muscles.\u00a0ClCs function as homodimers, allowing chloride and other anions to be conducted through each single protopore. Gating of the single monomer is fast while the common gate to open and close the pores simultaneously is slow. The channel gating can be modulated by intracellular\u00a0and extracellular chloride as well as pH.\u00a0ClC proteins mediate chloride flux across cellular\u00a0membranes in most cell types and participate in maintenance of resting membrane potential. Plasma\u00a0membrane chloride channels play an important role\u00a0in reducing muscle excitability. ClC-1 contributes to\u00a0membrane repolarization and stabilizes the membrane\u00a0voltage in skeletal muscle. Experimental block of the\u00a0chloride conductance mediated by ClC-1 facilitated\u00a0muscle hyperexcitability, manifested as myotonia.\u00a0Here we present data conducted on the SyncroPatch 384 showing characteristic biophysical\u00a0properties and pharmacology of hClC-1\u00a0expressed in CHO cells. We applied voltage protocols\u00a0including various test potentials to study the\u00a0voltage dependence of compounds. In order to\u00a0investigate compound binding properties we used\u00a0the internal perfusion system of the SyncroPatch 384\u00a0for direct application of anthracene-9-carboxylic acid\u00a0(9-AC) to the intracellular environment. Moreover, we\u00a0also investigated the effect of 9-AC and niflumic acid\u00a0(NFA) when they are applied from the extracellular side.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[42],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[],"target_categories":[61],"target_families":[372],"targets":[139],"resource_keyword":[],"class_list":["post-6856","resource_library","type-resource_library","status-publish","hentry","products_tags-syncropatch-384","resource_type-application-note","application_areas-ion-channels","target_categories-voltage-gated-ion-channels","target_families-clc-channels","targets-clc"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6856","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6856"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6856"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6856"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6856"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6856"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6856"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6856"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6856"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6856"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}