{"id":6846,"date":"2023-02-17T07:18:02","date_gmt":"2023-02-17T07:18:02","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/cav1-2-stability-and-pharmacology-of-cav1-2-channels-on-nanions-syncropatch-384pe\/"},"modified":"2023-02-17T07:18:02","modified_gmt":"2023-02-17T07:18:02","slug":"cav1-2-stability-and-pharmacology-of-cav1-2-channels-on-nanions-syncropatch-384pe","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/cav1-2-stability-and-pharmacology-of-cav1-2-channels-on-nanions-syncropatch-384pe\/","title":{"rendered":"CaV1.2 &#8211; &#8220;Stability and Pharmacology of CaV1.2 Channels on Nanion\u2019s SyncroPatch 384PE&#8221;"},"content":{"rendered":"<p>The CaV1.2 channel is a voltage-gated calcium channel (VGCC) expressed in a variety of mammalian tissues and is essential for multiple processes including CNS function, cardiac and smooth muscle contraction and Ca<sup>2+<\/sup>-selective pore, contains the voltage sensor and many of the binding sites for regulatory modulators and drugs and accessory subunits \u03b12\u03b4, \u03b2 and \u03b3 which are involved in anchorage, trafficking and regulatory functions. The CaV1.2 channel contains the alpha-1C subunit, encoded by the CACNA1C gene on the human chromosome 12p133. Mutations in the L-type Ca<sup>2+<\/sup>\u00a0channels have been associated with inherited arrhythmic disorders such as Timothy, Brugada and early repolarization syndromes. In addition, in the light of the CiPA initiative, the L-type channel is likely to become an important target for cardiac safety testing. Here we present high quality data with reliable pharmacology on CaV1.2 expressing CHO cells at a high throughput collected on the SyncroPatch 384PE. Current-voltage plots and concentration response curves for the compounds nifedipine and verapamil are shown. The IC<sub>50<\/sub>\u00a0values for these compounds are within the expected range and success rates of >70% for completed experiments were recorded. Importantly, CaV1.2 recorded on the Syncro- Patch 384PE exhibited stable peak amplitudes during the course of the experiment and displayed little or no rundown.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[42],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[54],"target_categories":[61],"target_families":[340],"targets":[91],"resource_keyword":[],"class_list":["post-6846","resource_library","type-resource_library","status-publish","hentry","products_tags-syncropatch-384","resource_type-application-note","application_areas-ion-channels","therapeutic_areas-cardiac-safety-cipa","target_categories-voltage-gated-ion-channels","target_families-cav-channels","targets-cav1-2"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6846","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6846"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6846"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6846"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6846"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6846"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6846"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6846"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6846"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6846"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}