{"id":6844,"date":"2023-02-17T07:17:57","date_gmt":"2023-02-17T07:17:57","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/cav1-2-high-throughput-pharmacology-of-cav1-2-channels-on-nanions-syncropatch-384pe\/"},"modified":"2023-02-17T07:17:59","modified_gmt":"2023-02-17T07:17:59","slug":"cav1-2-high-throughput-pharmacology-of-cav1-2-channels-on-nanions-syncropatch-384pe","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/cav1-2-high-throughput-pharmacology-of-cav1-2-channels-on-nanions-syncropatch-384pe\/","title":{"rendered":"CaV1.2 &#8211; &#8220;High Throughput Pharmacology of CaV1.2 Channels on Nanion\u2019s SyncroPatch 384PE&#8221;"},"content":{"rendered":"<p>The CaV1.2 channel is a voltage-gated calcium\u00a0channel (VGCC) expressed in a variety of mammalian tissues and is essential for multiple processes including CNS function, cardiac and smooth muscle contraction and neuroendocrine regulation. VGCC are composed of 4 subunits, which include an alpha 1 subunit which forms the\u00a0Ca<sup>2+<\/sup>-selective pore, contains the voltage sensor and many of the binding sites for regulatory modulators and drugs and accessory subunits \u03b12\u03b4, \u03b2 and \u03b3 which are involved in\u00a0anchorage, trafficking and regulatory functions. The\u00a0CaV1.2 channel contains the alpha-1C subunit, encoded by the CACNA1C gene on the human chromosome\u00a012p13. Mutations in the L-type Ca<sup>2+<\/sup>\u00a0channels have been associated with inherited arrhythmic disorders such as Timothy, Brugada and early repolarization syndromes. In addition, in the light of the CiPA initiative, the L-type\u00a0channel is likely to become an important target for cardiac safety testing.\u00a0Here we present high quality data with reliable\u00a0pharmacology on CaV1.2 expressing HEK cells at a high throughput collected on the SyncroPatch 384PE. Current-voltage plots and concentration response curves for the compounds nifedipine\u00a0and verapamil are shown. The IC<sub>50<\/sub>\u00a0values for these\u00a0compounds are within the expected range and\u00a0success rates of >75% for completed experiments were\u00a0recorded. Importantly, CaV1.2 recorded on the SyncroPatch\u00a0384PE exhibited stable peak amplitude during the course of the experiment and displayed little or no rundown.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[42],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[54],"target_categories":[61],"target_families":[340],"targets":[91],"resource_keyword":[],"class_list":["post-6844","resource_library","type-resource_library","status-publish","hentry","products_tags-syncropatch-384","resource_type-application-note","application_areas-ion-channels","therapeutic_areas-cardiac-safety-cipa","target_categories-voltage-gated-ion-channels","target_families-cav-channels","targets-cav1-2"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6844","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6844"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6844"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6844"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6844"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6844"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6844"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6844"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6844"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6844"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}