{"id":6826,"date":"2023-02-17T07:11:52","date_gmt":"2023-02-17T07:11:52","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/cic-electrophysiological-recordings-of-the-h-cl-exchanger-ec-clc-on-the-surfe2r-n1\/"},"modified":"2023-02-17T07:11:52","modified_gmt":"2023-02-17T07:11:52","slug":"cic-electrophysiological-recordings-of-the-h-cl-exchanger-ec-clc-on-the-surfe2r-n1","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/cic-electrophysiological-recordings-of-the-h-cl-exchanger-ec-clc-on-the-surfe2r-n1\/","title":{"rendered":"CIC &#8211; &#8220;Electrophysiological recordings of the H+\/ Cl- exchanger Ec-ClC on the SURFE2R N1&#8221;"},"content":{"rendered":"<p>ClCs are a family of chloride ion channels and transporters with important physiological roles including regulation of the membrane potential, transepithelial salt transport and ion homeostasis1,2. To date, 9 members of the ClC family have been identified in mammals1,2, the first 4 (ClC-1, ClC-2, ClC-Ka, and ClC-Kb) are located on the plasma\u00a0membrane where they act as chloride ion channels\u00a0whereas the remaining 5 are located in intracellular organelles (ClC-3-7) and are chloride-proton exchangers1-3. These transporters are important for endosome, lysosome and synaptic vesicle acidification1,2, and mutations in, e.g. ClC-5 underlie the rare chronic kidney disorder, Dent\u2019s Disease, and mutations in ClC-7 underlie osteopetrosis\u00a02,4, a rare inherited bone hardening disorder. Given their ubiquitous expression and importance in physiological\u00a0processes, they are important potential drug targets.ClC from Escherichia coli (Ec-ClC or ClC-ec1) is closely ClC from Escherichia coli (Ec-ClC or ClC-ec1) is closely related to its mammalian counterparts and is a Cl-\/H+\u00a0exchanger3,5. It transports 2 Cl- into the cell, coupled to the efflux of 1 H+3,5. Therefore Ec-ClC is an electrogenic transporter, generating a net charge flow. In E. coli, the Ec-ClC mediates acid resistance of enteric bacteria by promoting H+\u00a0extrusion1,5,6. Here we present Ec-ClC activity measurements on the SURFE2R N1 instrument using proteoliposomes reconstituted with purified Ec-ClC at different lipid-to-protein ratios.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[36],"resource_type":[90],"application_areas":[50,108],"therapeutic_areas":[],"target_categories":[61,60],"target_families":[343,372],"targets":[139],"resource_keyword":[],"class_list":["post-6826","resource_library","type-resource_library","status-publish","hentry","products_tags-surfe2r-n1","resource_type-application-note","application_areas-ion-channels","application_areas-transporters","target_categories-voltage-gated-ion-channels","target_categories-transporters","target_families-exchanger","target_families-clc-channels","targets-clc"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6826","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6826"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6826"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6826"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6826"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6826"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6826"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6826"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6826"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6826"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}