{"id":6808,"date":"2023-02-17T07:05:22","date_gmt":"2023-02-17T07:05:22","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/trpm8-cold-activation-of-trpm8-using-the-external-perfusion-system-and-the-port-a-patch\/"},"modified":"2023-02-17T07:05:22","modified_gmt":"2023-02-17T07:05:22","slug":"trpm8-cold-activation-of-trpm8-using-the-external-perfusion-system-and-the-port-a-patch","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/trpm8-cold-activation-of-trpm8-using-the-external-perfusion-system-and-the-port-a-patch\/","title":{"rendered":"TRPM8 &#8211; Cold activation of TRPM8 using the External Perfusion System and the Port-a-Patch"},"content":{"rendered":"<p>TRPM8 is a member of the transient receptor potential\u00a0channel (TRP) family. TRPM8 is known to be a thermosensitive\u00a0channel, activated by cold temperatures\u00a0(below ~25\u02daC) and ligands such as menthol, Eucalyptol\u00a0and icilin1-4. It belongs to the melastatin subfamily of\u00a0TRP channels5\u00a0and shows an outward rectification with a\u00a0relatively high permeability for calcium ions and\u00a0little selectivity between monovalent cations. Menthol,\u00a0a secondary alcohol produced by the peppermint\u00a0herb, Mentha piperita, is widely used in the food and\u00a0pharmaceutical industries as a cooling\/soothing\u00a0compound and odorant.Here we present data of hTRPM8 collected on the Port-a-Patch using cooled solution via the External Perfusion System. Cold activated hTRPM8 was blocked by capsazepine with an IC<sub>50<\/sub>\u00a0in good agreement with the literature.Here we present data of hTRPM8 collected on the Port-a-Patch using cooled solution via the External Perfusion System. Cold activated hTRPM8 was blocked by capsazepine with an IC<sub>50<\/sub>\u00a0in good agreement with the literature.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[55,56],"target_categories":[337,338],"target_families":[339],"targets":[122],"resource_keyword":[],"class_list":["post-6808","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","resource_type-application-note","application_areas-ion-channels","therapeutic_areas-cns","therapeutic_areas-pain","target_categories-ligand-gated-ion-channels","target_categories-other-ion-channels","target_families-trp-channels","targets-trpm8"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6808","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6808"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6808"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6808"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6808"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6808"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6808"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6808"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6808"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6808"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}