{"id":6796,"date":"2023-02-17T07:05:04","date_gmt":"2023-02-17T07:05:04","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/kv1-3-automated-internal-perfusion-of-jurkat-cells-expressing-kv1-3-channels\/"},"modified":"2023-02-17T07:05:04","modified_gmt":"2023-02-17T07:05:04","slug":"kv1-3-automated-internal-perfusion-of-jurkat-cells-expressing-kv1-3-channels","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/kv1-3-automated-internal-perfusion-of-jurkat-cells-expressing-kv1-3-channels\/","title":{"rendered":"KV1.3 &#8211; &#8220;Automated internal perfusion of Jurkat cells expressing KV1.3 channels&#8221;"},"content":{"rendered":"<p>KV1.3 is a voltage-gated potassium channel which plays a\u00a0role in human T cell activation and proliferation, cell-mediated\u00a0cytotoxicity and volume regulation. It is therefore\u00a0an important target for the therapeutic control of T cellresponses. The KV1.3 channel is endogenously expressed\u00a0in Jurkat cells, an immortalised T lymphocyte cell line. The\u00a0channel is activated by membrane depolarisation at voltages\u00a0typically more positive than -40 mV with rapid activation\u00a0kinetics and slower inactivation.\u00a0In common with many other voltage-gated potassium\u00a0channels, KV1.3 can be blocked by replacing K<sup>+<\/sup>\u00a0with Cs+\u00a0ions in the pipette solution. Using Nanion\u2019s Internal Perfusion\u00a0System, we were able to record stable KV1.3 currents\u00a0from Jurkat cells and reliably block this potassium current\u00a0by perfusing the internal side of the chip with a Cs+-containing\u00a0solution. The KV1.3 mediated current could also be\u00a0completely recovered by perfusing the inside of the chip\u00a0with K<sup>+<\/sup>-containing solution.\u00a0KV1.3 mediated currents can also be blocked by internal\u00a0perfusion with tetraethylammonium (TEA). We present\u00a0data here showing block of KV1.3 by increasing concentrationsof TEA using the Internal Perfusion System. The IC<sub>50<\/sub>\u00a0that we obtained for TEA applied internally was in good\u00a0agreement with the literature.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[45],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[],"target_categories":[61],"target_families":[333],"targets":[115],"resource_keyword":[],"class_list":["post-6796","resource_library","type-resource_library","status-publish","hentry","products_tags-port-a-patch","resource_type-application-note","application_areas-ion-channels","target_categories-voltage-gated-ion-channels","target_families-kv-channels","targets-kv1-3"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6796","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6796"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6796"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6796"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6796"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6796"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6796"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6796"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6796"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6796"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}