{"id":6776,"date":"2023-02-17T06:59:35","date_gmt":"2023-02-17T06:59:35","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/trpv3-heat-activation-of-trpv3-on-nanions-patchliner\/"},"modified":"2023-12-05T11:20:10","modified_gmt":"2023-12-05T11:20:10","slug":"trpv3-heat-activation-of-trpv3-on-nanions-patchliner","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/trpv3-heat-activation-of-trpv3-on-nanions-patchliner\/","title":{"rendered":"TRPV3 &#8211; &#8220;Heat activation of TRPV3 on Nanion&#8217;s Patchliner&#8221;"},"content":{"rendered":"<p>The transient receptor potential cation channel,\u00a0subfamily V, member 3 (TRPV3), is a ligand-gated, nonselective\u00a0cation channel first described in 2002. It\u00a0exhibits 43% sequence identity to TRPV1. Although\u00a0TRPV3 has been detected immunologically in the CNS\u00a0and suggested to be often co-localized with TRPV1, it is\u00a0found more robustly in keratinocytes in skin and, given it\u2019s\u00a0threshold for temperature activation of &gt;34\u02daC, it has\u00a0been speculated that TRPV3 may act in co-operation\u00a0with sensory afferents to perceive warmth and signal\u00a0elevated temperature. TRPV3 can also be activated by\u00a0the ligand 2-Aminoethoxydiphenyl borate (2-APB). The\u00a0TRPV3 channel, along with other TRPV channels, may\u00a0play an important role in chronic pain and, therefore, is\u00a0receiving increasing attention as a potential therapeutic\u00a0candidate for the treatment of chronic pain.\u00a0Here we present data collected on a 4-channel Patchliner\u00a0with temperature control showing the potential use of the\u00a0Patchliner to record TRPV3 currents activated by 2-APB\u00a0or heat. As previously reported, TRPV3 currents sensitize\u00a0to repeated applications of 2-APB or heat, a phenomenon\u00a0we also observed. At low concentrations of 2-APB,\u00a0the currents were primarily outwardly rectifying but at\u00a0higher concentrations and with prolonged exposure they\u00a0often became dual rectifying (data not shown). This is\u00a0also in good agreement with the literature. In contrast,\u00a0the temperature-activated responses were always outwardly\u00a0rectifying with little inward current. The inward currents\u00a0activated by 2-APB could be blocked by ruthenium\u00a0red (RR) as expected.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[44],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[55,56],"target_categories":[337,338],"target_families":[339],"targets":[121],"resource_keyword":[],"class_list":["post-6776","resource_library","type-resource_library","status-publish","hentry","products_tags-patchliner","resource_type-application-note","application_areas-ion-channels","therapeutic_areas-cns","therapeutic_areas-pain","target_categories-ligand-gated-ion-channels","target_categories-other-ion-channels","target_families-trp-channels","targets-trpv3"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6776","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6776"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6776"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6776"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6776"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6776"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6776"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6776"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6776"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6776"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}