{"id":6733,"date":"2023-02-17T06:58:32","date_gmt":"2023-02-17T06:58:32","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/ampa-receptor-glua2-activation-potentiation-and-inhibition-of-ampa-receptors-on-the-patchliner\/"},"modified":"2023-12-05T09:47:52","modified_gmt":"2023-12-05T09:47:52","slug":"ampa-receptor-glua2-activation-potentiation-and-inhibition-of-ampa-receptors-on-the-patchliner","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/ampa-receptor-glua2-activation-potentiation-and-inhibition-of-ampa-receptors-on-the-patchliner\/","title":{"rendered":"AMPA receptor (GluA2) &#8211; &#8220;Activation, potentiation and inhibition of AMPA receptors on the Patchliner&#8221;"},"content":{"rendered":"<p>AMPA receptors are cation-permeable ionotropic\u00a0glutamate receptors of the non-NMDA receptor subfamily.\u00a0To date four subunits, GluA1-4, have been identified\u00a0which are of similar size (approx. 900 kDa) and\u00a0share 68-73% amino acid sequence identity. The human\u00a0GluA2 subunit is encoded by the GRIA2 gene located on\u00a0the 4q32-33 chromosome. Each of the 4 subunits has four\u00a0distinct domains: an extracellular amino acid terminal\u00a0domain (ATD); the extracellular ligand binding domain\u00a0(LBD); the transmembrane domain (TMD) with 3 transmembrane\u00a0segments (M1, M3 and M4) and 1 cytoplasmic\u00a0facing re-entrant loop (M2); and an intracellular carboxyterminal\u00a0domain. The functional receptor exists as a\u00a0tetramer, either as homomers or heteromers (GluA1 and\u00a0GluA4). The vast majority of excitatory fast synaptic\u00a0transmission in the mammalian central nervous system is\u00a0mediated by AMPA receptors of differing subunit\u00a0combinations. It is well known that glutamate is a\u00a0neurotoxin and it is proposed that overactivation of\u00a0ionotropic glutamate receptors may underlie many\u00a0neurodegenerative disorders such as ischemic\u00a0stroke, epilepsy, Parkinson\u2019s and dementia,\u00a0amongst others. Enhancement of AMPA receptor\u00a0activation by, for example, BDNF, has been\u00a0proposed to have beneficial effects on learning and\u00a0memory and has potential therapeutic value in the treatment\u00a0of depression, Huntington\u2019s and\u00a0 Parkinson\u2019s diseases.\u00a0Here we present data collected on an 8-channel Patchliner\u00a0showing recordings of GluA2-mediated currents.\u00a0Glutamate activated GluA2 receptors with an EC<sub>50<\/sub>\u00a0similar\u00a0to those reported in the literature. CNQX inhibited and\u00a0LY404187 enhanced GluA2-mediated responses.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[44],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[55],"target_categories":[337],"target_families":[355],"targets":[104],"resource_keyword":[],"class_list":["post-6733","resource_library","type-resource_library","status-publish","hentry","products_tags-patchliner","resource_type-application-note","application_areas-ion-channels","therapeutic_areas-cns","target_categories-ligand-gated-ion-channels","target_families-ionotropic-glutamate-receptors","targets-glua2"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6733","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6733"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6733"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6733"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6733"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6733"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6733"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6733"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6733"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6733"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}