{"id":6729,"date":"2023-02-17T06:58:27","date_gmt":"2023-02-17T06:58:27","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/acetylcholine-receptor-alpha-3-beta-4-nicotinic-a3b4-receptors-recorded-on-nanions-patchliner\/"},"modified":"2023-04-14T12:28:15","modified_gmt":"2023-04-14T12:28:15","slug":"acetylcholine-receptor-alpha-3-beta-4-nicotinic-a3b4-receptors-recorded-on-nanions-patchliner","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/acetylcholine-receptor-alpha-3-beta-4-nicotinic-a3b4-receptors-recorded-on-nanions-patchliner\/","title":{"rendered":"Acetylcholine Receptor Alpha 3 Beta 4 &#8211; &#8221; Nicotinic a3b4 receptors recorded on Nanion&#8217;s Patchliner&#8221;"},"content":{"rendered":"<p>The nicotinic acetylcholine receptor (nAChR) is a member of the ligand-gated ion channel superfamily which includes GABAA, 5HT3, NMDA and glycine receptors. It is a cation-permeable ion channel activated by the neurotransmitter acetylcholine and the natural alkaloid, nicotine. Neuronal nAChR are pentameric and functional channels are formed from a repertoire of nine \u03b1 (\u03b12 to \u03b110) and three \u03b2 subunits (\u03b22 to \u03b24). Most nAChR exist as heteromers with the stoichiometry 2\u03b1 to 3\u03b2, however some \u03b1 subunits function as homomers, these being \u03b17 or \u03b19. nAChR have been proposed to play a role in many neurological disorders such as Alzheimer\u2019s Disease, Parkinson\u2019s, Tourette\u2019s Syndrome and depression. mRNA for the \u03b13 and \u03b24 subunits is found in the mammalian CNS and PNS, in particular automomic ganglion cells and chromaffin cells. nAChR containing the \u03b13 subunit are essential for mediating fast synaptic\u00a0 transmission in the autonomic nervous system and is essential for survival. Block of nicotinic \u03b13\u03b24 receptors by methadone has also been suggested to play a potential role in analgesia. Here we present data collected on a 4- or 8-channel Patchliner showing the potential use of the Patchliner to record nAChR \u03b13\u03b24 currents activated by nicotine. Nicotine activated \u03b13\u03b24 receptors in a concentration dependent manner with an EC<sub>50<\/sub>\u00a0similar to those reported in the literature. Nicotinic \u03b13\u03b24 receptors could be repetitively activated by nicotine and blocked by mecamylamine, a ganglionic blocker with clinically relevant hypotensive actions, with an IC<sub>50<\/sub>\u00a0in good agreement with the literature.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[44],"resource_type":[90],"application_areas":[50],"therapeutic_areas":[56],"target_categories":[337],"target_families":[360],"targets":[188],"resource_keyword":[],"class_list":["post-6729","resource_library","type-resource_library","status-publish","hentry","products_tags-patchliner","resource_type-application-note","application_areas-ion-channels","therapeutic_areas-pain","target_categories-ligand-gated-ion-channels","target_families-nicotinic-acetylcholine-receptors","targets-achr-3-4"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6729","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6729"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6729"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6729"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6729"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6729"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6729"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6729"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6729"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6729"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}