{"id":6713,"date":"2023-02-17T06:49:56","date_gmt":"2023-02-17T06:49:56","guid":{"rendered":"https:\/\/beta.nanion.de\/resource_library\/hepatocytes-toxicity-analysis-in-expanded-upcyte-liver-cells-on-nanions-cardioexcyte-96\/"},"modified":"2023-02-17T06:49:56","modified_gmt":"2023-02-17T06:49:56","slug":"hepatocytes-toxicity-analysis-in-expanded-upcyte-liver-cells-on-nanions-cardioexcyte-96","status":"publish","type":"resource_library","link":"https:\/\/nanion.com\/ja\/resource_library\/hepatocytes-toxicity-analysis-in-expanded-upcyte-liver-cells-on-nanions-cardioexcyte-96\/","title":{"rendered":"Hepatocytes &#8211; Toxicity analysis in expanded upcyte\u00ae liver cells on Nanion\u2019s CardioExcyte 96"},"content":{"rendered":"<p>Drug induced hepatic toxicity is one of the main reasons\u00a0for regulatory actions and market withdrawals in the last\u00a050 years [1]. In Europe and the USA, the major cause\u00a0of acute liver failure is indeed Drug Induced Liver Injury\u00a0(DILI) [2]. Current existing in vitro models employed to\u00a0predict DILI mostly focus on hepatocytes. Other cell types\u00a0of the liver, such as liver sinusoidal endothelial cells (LSECs),\u00a0are often overlooked. It is thus questionable whether,\u00a0hepatotoxicity can be sufficiently predicted by analyzing\u00a0hepatocytes only. LSECs are highly specialized endothelial\u00a0cells forming the hepatic sinusoidal wall. Besides their high\u00a0endocytic potential, LSECs have been demonstrated to\u00a0markedly contribute to liver homeostasis, immunity, and\u00a0may partially explain unexpected hepatotoxicity of\u00a0selected drug candidates.\u00a0Several reports in the literature have highlighted a high\u00a0sensitivity of LSECs towards hepatotoxic drugs [3]. It\u00a0has been suggested that LSECs act as an early direct\u00a0target for acetaminophen (paracetamol) induced\u00a0toxicity, causing early swelling and loss of uptake activity\u00a0and fenestrations before effects on hepatocytes are\u00a0observed [4]. LSECs are further important cellular targets\u00a0during sinusoidal obstruction syndrome (a distinctive\u00a0and potentially fatal form of hepatic injury that occurs\u00a0predominantly, if not only, after drug or toxin exposure).\u00a0The use of primary LSECs for comprehensive in vitro studies\u00a0is compromised by poor cell yields, rapid dedifferentiation,\u00a0contamination with other endothelial cells, and limited\u00a0proliferation after isolation [5]. In this study upcyte\u00ae LSECs\u00a0have been developed as a complementary tool to\u00a0predict hepatotoxicity, uptake and drug interactions. In\u00a0addition to conventional toxicity readouts such as ATP\u00a0levels, upcyte\u00ae LSECs have been successfully tested using\u00a0an impedance based system.<\/p>\n","protected":false},"featured_media":0,"template":"","products_tags":[34],"resource_type":[90],"application_areas":[53,77],"therapeutic_areas":[290],"target_categories":[],"target_families":[],"targets":[],"resource_keyword":[],"class_list":["post-6713","resource_library","type-resource_library","status-publish","hentry","products_tags-cardioexcyte-96","resource_type-application-note","application_areas-assay-development-and-validation","application_areas-cell-ipscs-profiling","therapeutic_areas-toxicology"],"acf":[],"_links":{"self":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library\/6713","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_library"}],"about":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/types\/resource_library"}],"wp:attachment":[{"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/media?parent=6713"}],"wp:term":[{"taxonomy":"products_tags","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/products_tags?post=6713"},{"taxonomy":"resource_type","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_type?post=6713"},{"taxonomy":"application_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/application_areas?post=6713"},{"taxonomy":"therapeutic_areas","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/therapeutic_areas?post=6713"},{"taxonomy":"target_categories","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_categories?post=6713"},{"taxonomy":"target_families","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/target_families?post=6713"},{"taxonomy":"targets","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/targets?post=6713"},{"taxonomy":"resource_keyword","embeddable":true,"href":"https:\/\/nanion.com\/ja\/wp-json\/wp\/v2\/resource_keyword?post=6713"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}